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Mouse Estradiol Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
1.88 pg/mL
Assay range
3.13-200 pg/mL
Sample type/volume
Urine
50 µL
Saliva
50 µL
Plasma
50 µL
Serum
50 µL
Hands-on time
20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Estradiol
Species (tested)
Mouse
Species (reported)
Mouse
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Mouse Estradiol (E2) ELISA quantitates E2 in Serum, Plasma, Urine, Saliva.
Principle of the method
The Mouse E2 solid phase competitive ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target based on competitive binding. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells, where Mouse E2 in the samples competes with the pre coated Mouse E2 for binding sites on the Horseradish Peroxidase (HRP) linked detection antibody. Excess conjugate and unbound sample or standard are washed away. A substrate solution is added that reacts with the enzyme antibody target complex to produce a measurable signal. The intensity of this signal, measured as optical density (OD) at a wavelength of 450±2 nm, is inversely proportional to the concentration of target present in the original specimen. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Estradiol (17 beta-estradiol) is a sex hormone. Estradiol is the predominant sex hormone present in females. It is also present in males, being produced as an active metabolic product of testosterone. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including the bones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : 17-Beta-Estradiol, 17-beta-HSD
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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