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Product Specifications
Analytical sensitivity
<5.63 pg/mL
Assay range
11.7-120,000 pg/mL
Sample type/volume
Plasma
50 µL
Saliva
25 µL
Supernatant
100 µL
Urine
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
12.4%
Intraassay CV
6.5%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent
Detection Antibody
AP Conjugate
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent
Detection Antibody
AP Conjugate
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Dry ice
Storage
-20°C
Protein name
Leukotriene B4
Protein family
Cytokines & Receptors
Species (tested)
Human, Pig
Assay kit format
Competitive ELISA
Label or dye
AP
About This Kit
The Multispecies Leukotriene B4 (LTB4) ELISA quantitates LTB4 in human saliva, urine, porcine plasma and tissue culture medium.
Principle of the method
This LTB4 competitive ELISA kit is an immunoassay for the quantitative determination of leukotriene B4 (LTB4) in biological fluids. The assay is based on the competition between LTB4 in the standard or sample and alkaline phosphatase conjugated LTB4 (LTB4-AP) for a limited amount of LTB4 monoclonal antibody bound to an anti-Rabbit IgG precoated 96-well plate. As the concentration of LTB4 in the sample increases, the amount of LTB4-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Leukotriene B4 (LTB4) is a leukotriene involved in inflammation. It is produced from leukocytes in response to inflammatory mediators and is able to induce the adhesion and activation of leukocytes on the endothelium, allowing them to bind and cross into the tissue. In neutrophils, LTB4 is a potent chemoattractant, and is able to induce the formation of reactive oxygen species and the release of lysosomal enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases : LTB4, C20H32O4
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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