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Product Specifications
Analytical sensitivity
18.75 pg/mL
Assay range
31.25-2,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
PTGES2
Protein family
Prostaglandin
Species (tested)
All
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The multispecies Prostaglandin E2 (PGE2) ELISA quantitates PGE2 in serum, plasma, and other biological fluids
Principle of the method:
The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Prostaglandin E2 (PGE2) independent of species. A PGE2 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to PGE2 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of PGE2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Prostoglandin E2 (PGE2) is a potent lipid mediator produced by metabolism of arachidonic acid via the cyclooxygenase (COX) pathway. Following cellular stimulation, arachidonic acid is hydrolyzed from phospholipids stores by one of a family of phospholipase A2 enzymes and converted to the unstable endoperoxide PGH2 by either COX 1 or COX 2. PGH2 is isomerized to PGE2 by one of 3 distinct enzymes microsomal PGES 1 (mPGES 1), mPGES 2, or a cytosolic enzyme, cPGES. PGE2 is active in a number of physiological systems and settings including inflammation, immune regulation, generation of fever, pain perception, protection of the gastric muscosa, fertility and parturition, as well as sodium and water retention. The effects of PGE2 are transduced by 4 subtypes of G protein coupled receptors designated EP1, EP2, EP3, EP4, with Kd values for PGE2 ranging from 1 10 nM. PGE2 is rapidly metabolized in vivo by the prostaglandin 15 dehydrogenase pathway (15 hydroxy PGDH) to the inactive metabolite 13,14 dihydro 15 keto PGE2. The half life of PGE2 in the circulatory system is ~30 seconds and normal plasma levels are 3 12 pg/mL
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : FLJ14038, MGC11289, RP11-395P17.2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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