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Product Specifications
Analytical sensitivity
80 pg/mL
Assay range
80-20,000 pg/mL
Sample type/volume
Supernatant
50 µL
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Osteopontin
Species (tested)
Rat
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Rat OPN quantitates rat OPN in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat OPN.
Target information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : 2b7, OSP, Spp-1, Spp1
Gene ID : (Rat) 25353
Gene symbol : Spp1
Protein Aliases : Bone sialoprotein 1, Osteopontin, Secreted phosphoprotein 1, Sialoprotein (osteopontin), SPP-1, urinar, HGNC:11255, immunoglobulin alpha 1 heavy chain constant region fusion protein, MGC110940, secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1), Secreted phosphoprotein 1 (SPP1), Bone sialoprotein 1 (BSPI/BSP1), CALPHA1 fusion, Early T lymphocyte activation 1 (ETA1)
UniProt ID (Rat) P08721
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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