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Invitrogen
Retinol Binding Protein ELISA Kit
Product Specifications
Analytical sensitivity
1.36 ng/mL
Assay range
7.8-1,000 ng/mL|7.8-1,000 ng/mL|7.8-1,000 ng/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Fecal Extract
50 µL
Urine
12.5 µL
Supernatant
50 µL
Hands-on time
45 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
5%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Anti-IgG coated 96 well plate
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Standard
Assay Buffer Concentrate
Capture Antibody
HRP-conjugated Antigen
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Plate Sealer
Shipping conditions
Dry ice
Storage
-20°C
Protein name
RBP1
Species (tested)
Human
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Retinol Binding Protein Competitive ELISA quantitates retinol binding protein (RBP) in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|Principle of the method: The Retinol Binding Protein Competitive ELISA research-use-only kit is designed to quantitatively measure RBP independent of species. An RBP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-sheep IgG antibodies. Anti-sheep RBP antibodies and RBP-peroxidase conjugate is added to the wells. The RBP-peroxidase conjugate and any RBP in the sample will compete to bind to the anti-sheep RBP antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound RBP-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of RBP present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CRABP-I, CRBP, CRBP1, CRBPI, RBP1, RBPC
Gene ID : (Human) 5947
Gene symbol : RBP1
Protein Aliases : C CRABP-I, RBP, retinol, retinol binding, Retinol Binding Protein, cellular retinol binding protein 1, Cellular retinol-binding protein, Cellular retinol-binding protein I, CRBP, CRBP-I, Retinol-binding protein 1, retinol-binding protein 1, cellular
UniProt ID (Human) P09455
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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