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Invitrogen
Serotonin Competitive ELISA Kit
Product Specifications
Analytical sensitivity
9.38 ng/mL
Assay range
15.63-1,000 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Serotonin
Protein family
Neurotransmitter
Species (tested)
All
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The multispecies Serotonin/5-Hydroxytryptamine (ST/5-HT) ELISA quantitates ST/5-HT in serum, plasma, and other biological fluids
Principle of the method:
The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Serotonin/5-Hydroxytryptamine (ST/5-HT) independent of species. A ST/5-HT standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to ST/5-HT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of ST/5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Serotonin (also known as 5-hydroxytryptamne or 5-HT) is a biochemical messenger and neurotransmitter synthesized from L-tryptophan. Serotonin has a range of functions, including regulation of gastric motility, and may be related to some psychiatric disorders including schizophrenia and depression. Serotoinin is widely distributed in the central and peripheral nervous system, and also in some non-nueral tissues such as platelets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : 5-HT, 5-hydroxytryptamne
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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