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Invitrogen
Vitamin D2/D3 Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
3.75 ng/mL
Assay range
6.25-400 ng/mL
Sample type/volume
Tissue Homogenate
50 µL
Serum
50 µL
Cell Lysate
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Vitamin D2/D3
Species (tested)
All
Species (reported)
All
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Vitamin D (VD) ELISA quantitates VD in serum, plasma and other biological fluids.
Principle of the method
The Competitive mulispecies ELISA research use only kit is designed to quantitatively measure Vitamin D (VD)independent of species . A Vitamin D standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve.Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Vitamin D is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Vitamin D in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Vitamin D2 and vitamin D3 are two forms of vitamin D, a group of fat-soluble vitamins that play crucial roles in maintaining overall health. Vitamin D2, also known as ergocalciferol, is derived from plant sources. It is produced when ultraviolet (UV) light converts ergosterol, a compound found in certain fungi and plants, into vitamin D2. Vitamin D3, also called cholecalciferol, is synthesized in the skin of humans and animals upon exposure to UVB light from the sun. It is the most biologically active form of vitamin D and is also found in certain animal-based food sources. Both vitamin D2 and D3 undergo similar metabolic processes in the liver and kidneys to be converted into their active form, known as calcitriol. Calcitriol acts as a hormone that regulates calcium and phosphorus metabolism in the body.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : Cholecalciferol, Ergocalciferol, Vitamin D2, vitamin D3, calciferol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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