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Invitrogen
Human DLL4 Fc Chimera Recombinant Protein
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Product Details
A42513
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human DLL4, amino acids Met1-Pro524 (Accession # NP_061947.1) with a C-terminal human IgG1 Fc-tag
Tag
Fc-tag
Molecular weight
81 kDa
Class
Recombinant
Type
Protein
Purity
≥95 % as determined by SDS-PAGE. ≥85 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized Mouse NOTCH1-His at 2 µg/mL (100 µL/well) can bind Human DLL4 Fc Chimera Recombinant Protein, the EC50 of human DLL4-hFc is 15-90 ng/mL.
2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1-8 µg/mL in the presence of 500 ng/mL recombinant human BMP2.
2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1-8 µg/mL in the presence of 500 ng/mL recombinant human BMP2.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Delta-like ligand 4 (DLL4), also know as Delta-4, is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formulation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: delta 4; delta ligand 4; delta-like 4 homolog; delta-like 4 protein; Delta-like protein 4; Delta4; Drosophila Delta homolog 4; MGC126344; notch ligand delta-2; notch ligand DLL4
Gene Aliases: AOS6; DLL4; hdelta2; UNQ1895/PRO4341
UniProt ID: (Human) Q9NR61
Entrez Gene ID: (Human) 54567
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