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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Proteins & Peptides ›
          • Her-2 (ErbB2) Proteins

          Invitrogen

          Human ErbB2 Fc Chimera Recombinant Protein

          View all (7) Her-2 (ErbB2) proteins

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          Cite Human ErbB2 Fc Chimera Recombinant Protein

          • Testing Data (1)
          Human ErbB2 Fc Chimera Protein in SDS-PAGE (SDS-PAGE)
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          Human ErbB2 Fc Chimera Protein in SDS-PAGE (SDS-PAGE)
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          FIGURE: 1 / 1

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          Human ErbB2 Fc Chimera Protein (A42494) in SDS-PAGE

          SDS-PAGE analysis of Human ErbB2 Fc Chimera (Product # A42494). The specification for purity shows: ≥ 90 % (SDS-PAGE), ≥ 90 % (SEC-HPLC). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Human ErbB2 Fc Chimera Protein in SDS-PAGE (SDS-PAGE)

          Product Details

          A42494

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          -

          Immunohistochemistry (IHC)

          Assay-dependent
          -

          ELISA (ELISA)

          Assay-dependent
          -

          Functional Assay (Functional)

          Assay-dependent
          -
          Product Specifications

          Species

          Human

          Expression System

          HEK293 cells

          Amino acid sequence

          Human ErbB2, amino acids Met1-Thr652 (Accession # NP_004439.2) with a C-terminal Fc-tag

          Tag

          Fc-tag

          Molecular weight

          96.1 kDa

          Class

          Recombinant

          Type

          Protein

          Purity

          ≥90 % as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC.

          Endotoxin concentration

          <1.0 EU/µg

          Activity

          1. Immobilized Human ErbB2 Fc Chimera Recombinant Protein at 2 µg/mL (100 µL/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 16-80 ng/mL.
          2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Purification

          purified

          Storage buffer

          PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

          Contains

          no preservative

          Storage conditions

          -20°C or -80°C if preferred

          Shipping conditions

          Ambient

          Product Specific Information

          Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

          Target Information

          ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: c-erb B2/neu protein; CD antigen CD340; CD340; erb-b2; erbb 2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; her 2; herstatin; human epidermal growth factor receptor 2; metas; Metastatic lymph node gene 19 protein; MLN 19; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; p185erbB2; phospho ErbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

          View more View less

          Gene Aliases: CD340; ERBB2; HER-2; HER-2/neu; HER2; MLN 19; MLN19; NEU; NGL; TKR1

          View more View less

          UniProt ID: (Human) P04626

          View more View less

          Entrez Gene ID: (Human) 2064

          View more View less

          It has to be done as per old AB suggested Products section.
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