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Product Details
450-47-10UG
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Expression System
Insect cells
Class
Recombinant
Type
Protein
Conjugate
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
450-47-100UG will be provided as 2 x 50 µg (450-47-50UG).
450-47-1MG will be provided as 20 x 50 µg (450-47-50UG).
450-47-250UG will be provided as 5 x 50 µg (450-47-50UG).
450-47-500UG will be provided as 5 x 100 µg (450-47-50UG).
Recombinant Human Furin is a 63.9 kDa protein, corresponding to residues 131 through 715 of the Furin precursor, plus a C-terminal His-tag.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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