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Invitrogen
Human HGFR (c-MET) His-tag Recombinant Protein
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Product Details
RP-88030
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human c-Met, amino acids Met1-Thr932 (Accession # NP_000236.2) with a C-terminal His-tag
Tag
His-tag
Molecular weight
45 kDa
Class
Recombinant
Type
Protein
Purity
>90% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized Cynomolgus HGF at 2 µg/mL (100 µL/well) can bind Human c-MET His, the EC50 of Human c-MET His is 20-120 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
20mM tris, pH 8.5, with 5% mannitol, 150mM NaCl, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (1099 µL) be added to the vial to prepare a stock solution of 0.91 mg/mL.
Concentration is measured by UV.
Target Information
MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; Tyrosine-protein kinase Met
Gene Aliases: AUTS9; c-Met; DFNB97; HGFR; MET; RCCP2
UniProt ID: (Human) P08581
Entrez Gene ID: (Human) 4233
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