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Human IL-27 Recombinant Protein, PeproTech®

Human IL-27 Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Human IL-27 Protein (200-38-10UG) in Functional

Bioassay analysis of Human IL-27 Recombinant Protein, PeproTech® (Product # 200-38-1MG).

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Product Details

200-38-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 13 publications 13 publications

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

HEK293 cells

Amino acid sequence

p28 subunit: FPRPPGRPQL SLQELRREFT VSLHLARKLL SEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP EBI3 subunit: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK

Molecular weight

47.8 kDa

Class

Recombinant

Type

Protein

Purity

≥ 90% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 range is 400-500 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human IL-27 produced from HEK293 cells is a non-disulfide-linked, heterodimeric protein composed of a 209 amino acid length EBI3 subunit and a 215 amino acid length IL-27-p28 subunit, for a total sequence length of 424 amino acid residues. The calculated molecular weight of the associated IL-27 subunits is 47.8 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Study on the Key Genes and Molecular Mechanisms of IL-27 Promoting Keratinocytes Proliferation Based on Transcriptome Sequencing.

Clinical, cosmetic and investigational dermatology

Wu Z,Yang Q,Xu K,Wu J,Yang B

200-38 was used in Cell Culture to promote keratinocyte proliferation by enhancing glycolysis, mitochondrial function, and mitochondrial fusion.

Wed Jun 14 00:00:00 UTC 2023

Identification of Keratinocyte Mitogens: Implications for Hyperproliferation in Psoriasis and Atopic Dermatitis.

JID innovations : skin science from molecules to population health

Niehues H,Rikken G,van Vlijmen-Willems IMJJ,Rodijk-Olthuis D,van Erp PEJ,Zeeuwen PLJM,Schalkwijk J,van den Bogaard EH

200-38 was used in In vitro experiments to investigate the mitogens involved in keratinocyte proliferation and stratification.

Sat Jan 01 00:00:00 UTC 2022

METTL3-mediated m⁶A RNA methylation promotes the anti-tumour immunity of natural killer cells.

Nature communications

Song H,Song J,Cheng M,Zheng M,Wang T,Tian S,Flavell RA,Zhu S,Li HB,Ding C,Wei H,Sun R,Peng H,Tian Z

Fri Sep 17 00:00:00 UTC 2021

A variant in IL6ST with a selective IL-11 signaling defect in human and mouse.

Bone research

Schwerd T,Krause F,Twigg SRF,Aschenbrenner D,Chen YH,Borgmeyer U,Müller M,Manrique S,Schumacher N,Wall SA,Jung J,Damm T,Glüer CC,Scheller J,Rose-John S,Jones EY,Laurence A,Wilkie AOM,Schmidt-Arras D,Uhlig HH

Wed Mar 06 00:00:00 UTC 2024

Enhanced STAT3 phosphorylation and PD-L1 expression in myeloid dendritic cells indicate impaired IL-27Ralpha signaling in type 1 diabetes.

Scientific reports

Parackova Z,Vrabcova P,Zentsova I,Kayserova J,Richtrova I,Sojka L,Stechova K,Sumnik Z,Sediva A

Thu Jan 16 00:00:00 UTC 2020

Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi.

Nature communications

Hong SP,Yang MJ,Cho H,Park I,Bae H,Choe K,Suh SH,Adams RH,Alitalo K,Lim D,Koh GY

Fri Aug 14 00:00:00 UTC 2020

The transcription factor Bhlhe40 is a switch of inflammatory versus antiinflammatory Th1 cell fate determination.

The Journal of experimental medicine

Yu F,Sharma S,Jankovic D,Gurram RK,Su P,Hu G,Li R,Rieder S,Zhao K,Sun B,Zhu J

Mon Jul 02 00:00:00 UTC 2018

ERAP1 deficient mice have reduced Type 1 regulatory T cells and develop skeletal and intestinal features of Ankylosing Spondylitis.

Scientific reports

Pepelyayeva Y,Rastall DPW,Aldhamen YA,O'Connell P,Raehtz S,Alyaqoub FS,Blake MK,Raedy AM,Angarita AM,Abbas AM,Pereira-Hicks CN,Roosa SG,McCabe L,Amalfitano A

Mon Aug 20 00:00:00 UTC 2018

JAK2-binding long noncoding RNA promotes breast cancer brain metastasis.

The Journal of clinical investigation

Wang S,Liang K,Hu Q,Li P,Song J,Yang Y,Yao J,Mangala LS,Li C,Yang W,Park PK,Hawke DH,Zhou J,Zhou Y,Xia W,Hung MC,Marks JR,Gallick GE,Lopez-Berestein G,Flores ER,Sood AK,Huang S,Yu D,Yang L,Lin C

Fri Dec 01 00:00:00 UTC 2017

JAK2-binding long noncoding RNA promotes breast cancer brain metastasis.

The Journal of clinical investigation

Wang S,Liang K,Hu Q,Li P,Song J,Yang Y,Yao J,Mangala LS,Li C,Yang W,Park PK,Hawke DH,Zhou J,Zhou Y,Xia W,Hung MC,Marks JR,Gallick GE,Lopez-Berestein G,Flores ER,Sood AK,Huang S,Yu D,Yang L,Lin C

Fri Dec 01 00:00:00 UTC 2017

A biallelic mutation in IL6ST encoding the GP130 co-receptor causes immunodeficiency and craniosynostosis.

The Journal of experimental medicine

Schwerd T,Twigg SRF,Aschenbrenner D,Manrique S,Miller KA,Taylor IB,Capitani M,McGowan SJ,Sweeney E,Weber A,Chen L,Bowness P,Riordan A,Cant A,Freeman AF,Milner JD,Holland SM,Frede N,Müller M,Schmidt-Arras D,Grimbacher B,Wall SA,Jones EY,Wilkie AOM,Uhlig HH

Mon Sep 04 00:00:00 UTC 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Wed Oct 11 00:00:00 UTC 2017

STAT3 Impairs STAT5 Activation in the Development of IL-9-Secreting T Cells.

Journal of immunology (Baltimore, Md. : 1950)

Olson MR,Verdan FF,Hufford MM,Dent AL,Kaplan MH

Fri Apr 15 00:00:00 UTC 2016

ERβ in CD4+ T Cells Is Crucial for Ligand-Mediated Suppression of Central Nervous System Autoimmunity.

Journal of immunology (Baltimore, Md. : 1950)

Aggelakopoulou M,Kourepini E,Paschalidis N,Panoutsakopoulou V

Wed Jun 15 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: cytokine receptor; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 p28 subunit; IL-27 subunit alpha; IL-27 subunit beta; IL-27-A; IL27 p28; IL27 subunit; IL27-A; IL35 subunit; ILN; Interleukin; interleukin 30; Interleukin-27 subunit alpha; Interleukin-27 subunit beta; Interleukin-30; M-IL-27 (p28); MGC71873; p28

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Gene Aliases: EBI3; IL-27; IL-27A; IL-27B; IL27; IL27A; IL27B; IL27p28; IL30; p28

Human IL-27 Recombinant Protein, PeproTech®

Human IL-27 Protein (200-38-10UG) in Functional

Product Details

200-38-10UG

Functional Assay (Functional)

In vitro Assay (IV)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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