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          • Proteins & Peptides ›
          • WNT1 Proteins
          Activate Wnt signaling with improved solubility. Try Gibco Wnt Surrogate-Fc Fusion Protein

          Gibco

          Human Wnt-1 Recombinant Protein

          View all (10) WNT1 proteins

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          Cite Human Wnt-1 Recombinant Protein

          Product Details

          PHC1804

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          -

          Immunohistochemistry (IHC)

          Assay-dependent
          -

          ELISA (ELISA)

          Assay-dependent
          -

          Functional Assay (Functional)

          Assay-dependent
          -

          Control (Ctrl)

          Assay-dependent
          -
          Product Specifications

          Species

          Human

          Expression System

          E. coli

          Amino acid sequence

          Human Wnt-1 recombinant protein contains 343 amino acids

          Molecular weight

          34.8 kDa

          Class

          Recombinant

          Type

          Protein

          Purity

          ≥98% by SDS-PAGE and HPLC

          Endotoxin concentration

          <0.1 ng/µg

          Activity

          ED50 = 1.5 - 2.5 ng/mL; determined by the dose-dependent enhancement of alkaline phosphatase production by murine ATDC5 cells treated with BMP-2 (200 ng/mL).

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Purification

          purified

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice

          Product Specific Information

          Carrier-Free

          Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.

          Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 6 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

          Target Information

          Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Proto-oncogene Int-1 homolog; Proto-oncogene Wnt-1; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)

          View more View less

          Gene Aliases: BMND16; INT1; OI15; WNT1

          View more View less

          UniProt ID: (Human) P04628

          View more View less

          Entrez Gene ID: (Human) 7471

          View more View less

          It has to be done as per old AB suggested Products section.
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