Gibco

Mouse MIP-1 beta (CCL4) Recombinant Protein, PeproTech®

Product Details

250-32-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 20 publications 20 publications

Miscellaneous PubMed (MISC)

View 7 publications 7 publications

Product Specifications

Species

Mouse

Published species

Hamster, Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN

Molecular weight

7.8 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human monocytes using a concentration range of 20.0-100.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine MIP-1beta is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aging-induced IL27Ra signaling impairs hematopoietic stem cells.

Blood

He H,Xu P,Zhang X,Liao M,Dong Q,Cong T,Tang B,Yang X,Ye M,Chang Y,Liu W,Wang X,Ju Z,Wang J

Thu Jul 09 00:00:00 UTC 2020

CCR5 deficiency impairs CD4⁺ T-cell memory responses and antigenic sensitivity through increased ceramide synthesis.

The EMBO journal

Martín-Leal A,Blanco R,Casas J,Sáez ME,Rodríguez-Bovolenta E,de Rojas I,Drechsler C,Real LM,Fabrias G,Ruíz A,Castro M,Schamel WW,Alarcón B,van Santen HM,Mañes S

Mon Aug 03 00:00:00 UTC 2020

Cytotoxic T cells swarm by homotypic chemokine signalling.

eLife

Galeano Niño JL,Pageon SV,Tay SS,Colakoglu F,Kempe D,Hywood J,Mazalo JK,Cremasco J,Govendir MA,Dagley LF,Hsu K,Rizzetto S,Zieba J,Rice G,Prior V,O'Neill GM,Williams RJ,Nisbet DR,Kramer B,Webb AI,Luciani F,Read MN,Biro M

Tue Oct 13 00:00:00 UTC 2020

Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance.

Nature communications

Meyer MA,Baer JM,Knolhoff BL,Nywening TM,Panni RZ,Su X,Weilbaecher KN,Hawkins WG,Ma C,Fields RC,Linehan DC,Challen GA,Faccio R,Aft RL,DeNardo DG

Wed Mar 28 00:00:00 UTC 2018

CCL4 enhances preosteoclast migration and its receptor CCR5 downregulation by RANKL promotes osteoclastogenesis.

Cell death & disease

Lee D,Shin KJ,Kim DW,Yoon KA,Choi YJ,Lee BNR,Cho JY

Tue May 01 00:00:00 UTC 2018

CCL4 enhances preosteoclast migration and its receptor CCR5 downregulation by RANKL promotes osteoclastogenesis.

Cell death & disease

Lee D,Shin KJ,Kim DW,Yoon KA,Choi YJ,Lee BNR,Cho JY

Tue May 01 00:00:00 UTC 2018

A molecular roadmap of the AGM region reveals BMPER as a novel regulator of HSC maturation.

The Journal of experimental medicine

McGarvey AC,Rybtsov S,Souilhol C,Tamagno S,Rice R,Hills D,Godwin D,Rice D,Tomlinson SR,Medvinsky A

Mon Dec 04 00:00:00 UTC 2017

IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.

Scientific reports

Thaney VE,O'Neill AM,Hoefer MM,Maung R,Sanchez AB,Kaul M

250-32 was used in In vitro experiments to illustrate the ability of exogenous IFNβ to mitigate HIVgp120-induced brain injury in rodents, acting as a neuroprotective agent.

Thu Apr 20 00:00:00 UTC 2017

IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.

Scientific reports

Thaney VE,O'Neill AM,Hoefer MM,Maung R,Sanchez AB,Kaul M

250-32 was used in In vitro experiments to illustrate the ability of exogenous IFNβ to mitigate HIVgp120-induced brain injury in rodents, acting as a neuroprotective agent.

Thu Apr 20 00:00:00 UTC 2017

Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.

Scientific reports

Singh K,Davies G,Alenazi Y,Eaton JRO,Kawamura A,Bhattacharya S

Tue Jun 27 00:00:00 UTC 2017

Systematic Screening of Chemokines to Identify Candidates to Model and Create Ectopic Lymph Node Structures for Cancer Immunotherapy.

Scientific reports

Yagawa Y,Robertson-Tessi M,Zhou SL,Anderson ARA,Mulé JJ,Mailloux AW

Wed Nov 22 00:00:00 UTC 2017

IL-5 triggers a cooperative cytokine network that promotes eosinophil precursor maturation.

Journal of immunology (Baltimore, Md. : 1950)

Fulkerson PC,Schollaert KL,Bouffi C,Rothenberg ME

Wed Oct 15 00:00:00 UTC 2014

Macrophage-secreted cytokines drive pancreatic acinar-to-ductal metaplasia through NF-κB and MMPs.

The Journal of cell biology

Liou GY,Döppler H,Necela B,Krishna M,Crawford HC,Raimondo M,Storz P

Mon Aug 05 00:00:00 UTC 2013

IL-27 receptor signaling regulates CD4+ T cell chemotactic responses during infection.

Journal of immunology (Baltimore, Md. : 1950)

Gwyer Findlay E,Villegas-Mendez A,de Souza JB,Inkson CA,Shaw TN,Saris CJ,Hunter CA,Riley EM,Couper KN

Wed May 01 00:00:00 UTC 2013

Tumor-infiltrating monocytic myeloid-derived suppressor cells mediate CCR5-dependent recruitment of regulatory T cells favoring tumor growth.

Journal of immunology (Baltimore, Md. : 1950)

Schlecker E,Stojanovic A,Eisen C,Quack C,Falk CS,Umansky V,Cerwenka A

Sat Dec 15 00:00:00 UTC 2012

A combination of local inflammation and central memory T cells potentiates immunotherapy in the skin.

Journal of immunology (Baltimore, Md. : 1950)

Fiorenza S,Kenna TJ,Comerford I,McColl S,Steptoe RJ,Leggatt GR,Frazer IH

Sat Dec 15 00:00:00 UTC 2012

IL-4 engagement of the type I IL-4 receptor complex enhances mouse eosinophil migration to eotaxin-1 in vitro.

PloS one

Heller NM,Gwinn WM,Donnelly RP,Constant SL,Keegan AD

Fri Jan 04 00:00:00 UTC 2013

An aberrant thymus in CCR5-/- mice is coupled with an enhanced adaptive immune response in fungal infection.

Journal of immunology (Baltimore, Md. : 1950)

Kroetz DN,Deepe GS

Sun May 15 00:00:00 UTC 2011

Pan-CC chemokine neutralization restricts splenocyte egress and reduces inflammation in a model of arthritis.

Journal of immunology (Baltimore, Md. : 1950)

Buatois V,Fagète S,Magistrelli G,Chatel L,Fischer N,Kosco-Vilbois MH,Ferlin WG

Sun Aug 15 00:00:00 UTC 2010

Docosahexaenoic acid prevents dendritic cell maturation and in vitro and in vivo expression of the IL-12 cytokine family.

Lipids in health and disease

Kong W,Yen JH,Vassiliou E,Adhikary S,Toscano MG,Ganea D

Mon Feb 01 00:00:00 UTC 2010

CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.

Blood

Achour L,Scott MG,Shirvani H,Thuret A,Bismuth G,Labbé-Jullié C,Marullo S

Thu Feb 26 00:00:00 UTC 2009

A novel recombinant fusion protein encoding a 20-amino acid residue of the third extracellular (E3) domain of CCR2 neutralizes the biological activity of CCL2.

Journal of immunology (Baltimore, Md. : 1950)

Izhak L,Wildbaum G,Zohar Y,Anunu R,Klapper L,Elkeles A,Seagal J,Yefenof E,Ayalon-Soffer M,Karin N

Wed Jul 01 00:00:00 UTC 2009

The chemokine receptor CCR5 plays a key role in the early memory CD8+ T cell response to respiratory virus infections.

Immunity

Kohlmeier JE,Miller SC,Smith J,Lu B,Gerard C,Cookenham T,Roberts AD,Woodland DL

Fri Jul 18 00:00:00 UTC 2008

Structure and function of A41, a vaccinia virus chemokine binding protein.

PLoS pathogens

Bahar MW,Kenyon JC,Putz MM,Abrescia NG,Pease JE,Wise EL,Stuart DI,Smith GL,Grimes JM

Tue Jan 01 00:00:00 UTC 2008

CXCL10 blockade protects mice from cyclophosphamide-induced cystitis.

Journal of immune based therapies and vaccines

Sakthivel SK,Singh UP,Singh S,Taub DD,Novakovic KR,Lillard JW

Tue Oct 28 00:00:00 UTC 2008

HIV-1 coreceptors CCR5 and CXCR4 both mediate neuronal cell death but CCR5 paradoxically can also contribute to protection.

Cell death and differentiation

Kaul M,Ma Q,Medders KE,Desai MK,Lipton SA

Thu Feb 01 00:00:00 UTC 2007

Galectin-3 regulates myofibroblast activation and hepatic fibrosis.

Proceedings of the National Academy of Sciences of the United States of America

Henderson NC,Mackinnon AC,Farnworth SL,Poirier F,Russo FP,Iredale JP,Haslett C,Simpson KJ,Sethi T

Tue Mar 28 00:00:00 UTC 2006

Critical role for CCR5 in the function of donor CD4+CD25+ regulatory T cells during acute graft-versus-host disease.

Blood

Wysocki CA,Jiang Q,Panoskaltsis-Mortari A,Taylor PA,McKinnon KP,Su L,Blazar BR,Serody JS

Tue Nov 01 00:00:00 UTC 2005

MIP-1alpha and MIP-1beta differentially mediate mucosal and systemic adaptive immunity.

Blood

Lillard JW,Singh UP,Boyaka PN,Singh S,Taub DD,McGhee JR

Sat Feb 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: ACT-2; ACT2; C-C motif chemokine 4; Immune activation protein 2; Macrophage inflammatory protein 1-beta; MGC104418; MGC126025; MGC126026; MIP-1 beta; MIP-1-beta; MIP1-beta; MIP1beta; Protein H400; RP23-320E6.8; SIS-gamma; small inducible cytokine A4; Small-inducible cytokine A4

Mouse MIP-1 beta (CCL4) Recombinant Protein, PeproTech®

Product Details

250-32-10UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (MISC)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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