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Product Details
RP-8632
Product Specifications
Species
Mouse
Expression System
E. coli
Amino acid sequence
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Molecular weight
18.4 kDa
Class
Recombinant
Type
Protein
Purity
≥95%
Endotoxin concentration
≤0.1 EU/µg
Activity
ED50 ≤20 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
Conjugate
Form
Lyophilized
Concentration
0.1 mg/mL
Purification
purified
Storage buffer
20mM sodium phosphate, pH 6.5
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Reconstitute in sterile water to 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Target Information
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
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Bioinformatics
Protein Aliases: C-kit ligand; cloud gray; DKFZp686F2250; grizzle-belly; H-SCF; Hematopoietic growth factor KL; Kit ligand; kit-ligand; M-SCF; Mast cell growth factor; MGF; SCF; Steel factor; Stem cell factor
Gene Aliases: blz; Clo; Con; contrasted; Gb; Kitl; Kitlg; Mgf; SCF; SF; Sl; SLF
UniProt ID: (Mouse) P20826
Entrez Gene ID: (Mouse) 17311
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