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          • Proteins & Peptides ›
          • Interferon alpha-2 Proteins

          Invitrogen

          Rat IFN-alpha 2 Recombinant Protein

          View all (7) Interferon alpha-2 proteins

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          Cite Rat IFN-alpha 2 Recombinant Protein

          Product Details

          131001

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          -

          ELISA (ELISA)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species

          Rat

          Expression System

          E. coli

          Molecular weight

          19 kDa

          Class

          Recombinant

          Type

          Protein

          Purity

          >95%

          Endotoxin concentration

          <1 EU/µg

          Activity

          EC50 ~ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394]As there is no international Rat IFN-alpha standard, units are determined with respect to the international reference standard for mouse interferon alpha/beta (mouse IFN-alpha/beta) provided by the National Institutes of Health.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Purification

          purified

          Storage buffer

          citric acid, pH 5, with NaCl, 0.125M trehalose

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Wet ice

          Product Specific Information

          Each blood donation used for interferon production tested negative for: HBsAg, HIV I-II, HCV and VDRL.

          After receipt, this product should be kept at 4°C while lyophilized and at -70°C after reconstitution in sterile distilled water for retention of full activity. After reconstitution store at -70°C in aliquots. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Further dilute only in buffers containing protein such as 0.1% bovine serum albumin (BSA).

          Target Information

          IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: IFN-a; IFN-alpha 1b; IFN-alpha-1/13; IFNA 2; Interferon; interferon alpha 1b; interferon alpha family, gene 2; interferon alpha-D; Interferon-alpha II; leIF D; RP11-354P17.2

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          Entrez Gene ID: (Rat) 298213

          View more View less

          It has to be done as per old AB suggested Products section.
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