Invitrogen

14-3-3 Pan Polyclonal Antibody

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12 Published Figures
12 References
View all (3) 14-3-3 Pan antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

51-0700

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
View 10 publications 10 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL
View 1 publication 1 publication

ELISA (ELISA)

0.1-1.0 µg/mL
-
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat, Xenopus

Published species

Bovine, Fruit fly, Human, Mouse, Plant

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 20 amino acid synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533894

Product Specific Information

This antibody is broadly reactive with members of the 14-3-3 family of proteins (~29-32 kDa). With the exception of a single conserved amino acid change, this sequence is identical in the delta and zeta isoforms. Three and four conserved amino acid differences are found in the tau and gamma isoforms, respectively.

Based on the sequence homology to the immunizing peptide, this the antibody could recognize the protein from C. elegans.

Target Information

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 14-3-3 alpha; 14-3-3 beta; 14-3-3 delta; 14-3-3 epsilon; 14-3-3 gamma; 14-3-3 protein beta; 14-3-3 protein beta-subtype; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha-A; 14-3-3 protein beta/alpha-B; 14-3-3 protein epsilon; 14-3-3 protein gamma; 14-3-3 protein gamma subtype; 14-3-3 protein gamma-A; 14-3-3 protein gamma-B; 14-3-3 protein gamma-subtype; 14-3-3 protein sigma; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; 14-3-3 protein zeta; 14-3-3 protein zeta/delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 sigma; 14-3-3 sigma protein; 14-3-3 tau; 14-3-3 theta; 14-3-3 zeta; 14-3-3d; 14-3-3E; 3-monooxgenase/tryptophan 5-monooxgenase activation protein, gamma polypeptide; 3-monooxgenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; 3-monooxygenase/tryptophan 5-monooxgenase activation protein gamma polypeptide; 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; brain protein 14-3-3, beta isoform; epididymis luminal protein 2; epididymis luminal protein 4; epididymis secretory protein Li 1; epididymis secretory protein Li 3; epididymis secretory protein Li 93; Epithelial cell marker protein 1; Factor activating exoenzyme S; FAS; KCIP-1; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; Mitochondrial import stimulation factor S1 subunit; MSF L; phospholipase A2; Prepronerve growth factor RNH-1; Protein 1054; Protein HS1; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; protein phosphatase 1, regulatory subunit 170; protein tau; protein, theta; SEZ-2; Stratifin; tryosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein gamma; tryosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein gamma polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, sigma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein gamma polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, gamma polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide

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Gene Aliases: 1110013I11Rik; 1300003C17Rik; 14-3-3; 14-3-3-zeta; 14-3-3beta; 14-3-3E; 14-3-3G; 14-3-3GAMMA; 14-3-3t; 14-3-3z; 14-3-3zeta; 1C5; 2700028P07Rik; AA409740; AI596267; AL022924; AU019196; AU020854; AU021156; BOS_11879; BOS_13300; BOS_14050; BOS_18528; BOS_22941; BOS_2359; D7Bwg1348e; Er; GW128; HEL-S-1; HEL-S-3; HEL-S-93; HEL2; HEL4; HME1; HS1; KCIP-1; MDCR; MDS; Mkrn3; Mme1; Msfs1; PPP1R170; R74690; SFN; XELAEV_18012695mg; XELAEV_18015318mg; XELAEV_18043238mg; XELAEV_18045999mg; YWHAA; YWHAB; ywhab-a; ywhab-b; YWHAD; YWHAE; YWHAG; ywhag-a; ywhag-b; YWHAQ; YWHAS; YWHAZ

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UniProt ID: (Bovine) Q0VC36, (Human) P31947, (Mouse) O70456, (Bovine) P68250, (Human) P31946, (Bovine) P62261, (Human) P62258, (Human) P61981, (Human) P27348, (Bovine) Q3SZI4, (Human) P63104, (Bovine) P63103, (Mouse) Q9CQV8, (Rat) P35213, (Mouse) P62259, (Rat) P62260, (Mouse) P61982, (Rat) P61983, (Rat) P68255, (Mouse) P68254, (Mouse) P63101, (Rat) P63102, (Xenopus) Q5XHK2, (Xenopus) Q8AVQ3, (Xenopus) Q6PCG0, (Xenopus) Q6NRY9

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Entrez Gene ID: (Bovine) 528453, (Human) 2810, (Mouse) 55948, (Rat) 313017, (Bovine) 286863, (Human) 7529, (Bovine) 282125, (Human) 7531, (Human) 7532, (Bovine) 286862, (Human) 10971, (Bovine) 768311, (Human) 7534, (Bovine) 287022, (Mouse) 54401, (Rat) 56011, (Mouse) 22627, (Rat) 29753, (Mouse) 22628, (Rat) 56010, (Rat) 25577, (Mouse) 22630, (Mouse) 22631, (Rat) 25578, (Xenopus) 399276, (Xenopus) 380457, (Xenopus) 379724, (Xenopus) 432005, (Xenopus) 100505434

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Function(s)
protein kinase binding protein domain specific binding phosphoprotein binding protein binding protein kinase C inhibitor activity cadherin binding involved in cell-cell adhesion molecular_function histone deacetylase binding phosphoserine binding transcription corepressor activity protein C-terminus binding enzyme binding protein complex binding potassium channel regulator activity MHC class II protein complex binding ubiquitin protein ligase binding ion channel binding poly(A) RNA binding protein heterodimerization activity protein kinase C binding insulin-like growth factor receptor binding receptor tyrosine kinase binding protein N-terminus binding 14-3-3 protein binding transcription factor binding identical protein binding actin binding enzyme regulator activity
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity release of cytochrome c from mitochondria negative regulation of cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage keratinocyte differentiation positive regulation of cell growth negative regulation of cysteine-type endopeptidase activity involved in apoptotic process keratinocyte proliferation positive regulation of protein export from nucleus histamine secretion by mast cell protein targeting protein targeting to mitochondrion regulation of cell death response to drug establishment of Golgi localization Golgi reassembly negative regulation of protein dephosphorylation positive regulation of catalytic activity negative regulation of transcription, DNA-templated cytoplasmic sequestering of protein protein heterooligomerization neuron migration hippocampus development cerebral cortex development regulation of membrane repolarization regulation of potassium ion transmembrane transporter activity negative regulation of peptidyl-serine dephosphorylation small GTPase mediated signal transduction substantia nigra development negative regulation of ion transmembrane transport membrane organization positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway keratinocyte development negative regulation of protein kinase activity DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest signal transduction regulation of epidermal cell division negative regulation of keratinocyte proliferation keratinization positive regulation of epidermal cell differentiation establishment of skin barrier negative regulation of protein serine/threonine kinase activity cell-cell adhesion platelet activation negative regulation of apoptotic process regulation of mRNA stability skin development regulation of cell cycle G2/M transition of mitotic cell cycle regulation of signal transduction cellular response to insulin stimulus regulation of neuron differentiation regulation of synaptic plasticity biological_process regulation of catalytic activity regulation of heart rate by hormone viral process hippo signaling intracellular signal transduction membrane repolarization during cardiac muscle cell action potential regulation of heart rate by cardiac conduction regulation of cellular response to heat MAPK cascade negative regulation of G-protein coupled receptor protein signaling pathway
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