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Invitrogen
14-3-3 beta/zeta Recombinant Rabbit Monoclonal Antibody (7Z4S5)
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FIGURE: 1 / 1
14-3-3 beta/zeta Antibody (MA5-42912) in WB
Western blot analysis of 14-3-3 beta/zeta in extracts of various cell lines. Samples were incubated with 14-3-3 beta/zeta Monoclonal antibody (Product # MA5-42912) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-42912
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7Z4S5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 14-3-3 beta/zeta (P31946)
Conjugate
Form
Liquid
Concentration
0.28 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2912053
Product Specific Information
Positive test controls include: HT-29, A-549, NIH/3T3, Jurkat, Mouse brain, Mouse kidney, Rat lung, Rat brain, Rat spleen.
Immunogen sequence: MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL
Target Information
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3 alpha; 14-3-3 delta; 14-3-3 protein beta; 14-3-3 protein beta-subtype; 14-3-3 protein beta/alpha; 14-3-3 protein zeta; 14-3-3 protein zeta/delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; 14-3-3d; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; brain protein 14-3-3, beta isoform; epididymis luminal protein 4; epididymis secretory protein Li 1; epididymis secretory protein Li 3; epididymis secretory protein Li 93; KCIP-1; Mitochondrial import stimulation factor S1 subunit; phospholipase A2; Prepronerve growth factor RNH-1; Protein 1054; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; SEZ-2; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; YWHAE
Gene Aliases: 1110013I11Rik; 1300003C17Rik; 14-3-3-zeta; 14-3-3z; 14-3-3zeta; AI596267; AL022924; AU020854; GW128; HEL-S-1; HEL-S-3; HEL-S-93; HEL4; HS1; KCIP-1; Msfs1; YWHAA; YWHAB; YWHAD; YWHAZ
UniProt ID: (Human) P31946, (Human) P63104, (Mouse) Q9CQV8, (Rat) P35213, (Mouse) P63101, (Rat) P63102
Entrez Gene ID: (Human) 7529, (Human) 7534, (Mouse) 54401, (Rat) 56011, (Mouse) 22631, (Rat) 25578
transcription corepressor activity
protein binding
protein C-terminus binding
enzyme binding
protein domain specific binding
protein complex binding
histone deacetylase binding
phosphoserine binding
phosphoprotein binding
cadherin binding involved in cell-cell adhesion
transcription factor binding
protein kinase binding
ubiquitin protein ligase binding
identical protein binding
poly(A) RNA binding
protein targeting
signal transduction
platelet activation
negative regulation of apoptotic process
regulation of mRNA stability
establishment of Golgi localization
membrane organization
Golgi reassembly
cell-cell adhesion
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
histamine secretion by mast cell
protein targeting to mitochondrion
regulation of cell death
response to drug
negative regulation of protein dephosphorylation
positive regulation of catalytic activity
negative regulation of transcription, DNA-templated
cytoplasmic sequestering of protein
protein heterooligomerization
MAPK cascade
viral process
hippo signaling
negative regulation of G-protein coupled receptor protein signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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