Proteintech

14-3-3 epsilon Polyclonal Antibody

14-3-3 epsilon Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

14-3-3 epsilon Antibody (11648-2-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using YWHAE antibody 11648-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

14-3-3 epsilon Antibody in Immunohistochemistry (Paraffin) (IHC (P))

14-3-3 epsilon Antibody in Western Blot (WB)

14-3-3 epsilon Antibody in Flow Cytometry (Flow)

14-3-3 epsilon Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11648-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

14-3-3 epsilon Fusion Protein Ag2247 (1-255 aa encoded by BC000179)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody was raised against full-length 14-3-3 epsilon.

Immunogen sequence: MDDREDLVY QAKLAEQAER YDEMVESMKK VAGMDVELTV EERNLLSVAY KNVIGARRAS WRIISSIEQK EENKGGEDKL KMIREYRQMV ETELKLICCD ILDVLDKHLI PAANTGESKV FYYKMKGDYH RYLAEFATGN DRKEAAENSL VAYKAASDIA MTELPPTHPI RLGLALNFSV FYYEILNSPD RACRLAKAAF DDAIAELDTL SEESYKDSTL IMQLLRDNLT LWTSDMQGDG EEQNKEALQD VEDENQ (1-255 aa encoded by B C000179)

Target Information

14-3-3 epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Cell biology and toxicology

Luo Q,Pan Y,Fu Q,Zhang X,Zhou S,Yu P,Tian H,Liu P,Chen S,Zhang H,Qin T

Sun Oct 01 00:00:00 UTC 2023

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Cell biology and toxicology

Luo Q,Pan Y,Fu Q,Zhang X,Zhou S,Yu P,Tian H,Liu P,Chen S,Zhang H,Qin T

Sun Oct 01 00:00:00 UTC 2023

Stabilization of 14-3-3 protein-protein interactions with Fusicoccin-A decreases alpha-synuclein dependent cell-autonomous death in neuronal and mouse models.

Neurobiology of disease

Vinueza-Gavilanes R,Bravo-González JJ,Basurco L,Boncristiani C,Fernández-Irigoyen J,Santamaría E,Marcilla I,Pérez-Mediavilla A,Luquin MR,Vales A,González-Aseguinolaza G,Aymerich MS,Aragón T,Arrasate M

Sat Jul 01 00:00:00 UTC 2023

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Haematologica

Michalik S,Siegerist F,Palankar R,Franzke K,Schindler M,Reder A,Seifert U,Cammann C,Wesche J,Steil L,Hentschker C,Gesell-Salazar M,Reisinger E,Beer M,Endlich N,Greinacher A,Völker U

Fri Apr 01 00:00:00 UTC 2022

LncRNA Gm16638-201 Inhibits the 14-3-3Ɛ Pathway in the Murine Prefrontal Cortex to Induce Depressive Behaviors.

Biological & pharmaceutical bulletin

Zhang T,Wang WL,Liu TJ,Lu S,Bian YC,Xiao R,Zhang CL

Mon Nov 07 00:00:00 UTC 2022

AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.

The EMBO journal

Zheng X,Xiao J,Jiang Q,Zheng L,Liu C,Dong C,Zheng Y,Ni P,Zhang C,Zhang F,Zhong R,Ding H,Wang Q,Qiu Y,Gao M,Ding J,Shen N,Wei B,Wang H

Tue Mar 15 00:00:00 UTC 2022

Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis.

Cell death & disease

Lou Y,Xu J,Zhang Y,Zhang W,Zhang X,Gu P,Zhong H,Wang H,Lu J,Han B

Wed Feb 10 00:00:00 UTC 2021

Role of the Chaperone Protein 14-3-3ε in the Regulation of Influenza A Virus-Activated Beta Interferon.

Journal of virology

Tam EH,Liu YC,Woung CH,Liu HM,Wu GH,Wu CC,Kuo RL

Mon Sep 27 00:00:00 UTC 2021

A Novel Heptapeptide, GPPGPAG Transfers to the Brain, and Ameliorates Memory Dysfunction and Dendritic Atrophy in Alzheimer's Disease Model Mice.

Frontiers in pharmacology

Tohda C,Kogure C,Nomoto K,de Toledo A,Yang X,Hirano E

Tue Jun 01 00:00:00 UTC 2021

A Novel Heptapeptide, GPPGPAG Transfers to the Brain, and Ameliorates Memory Dysfunction and Dendritic Atrophy in Alzheimer's Disease Model Mice.

Frontiers in pharmacology

Tohda C,Kogure C,Nomoto K,de Toledo A,Yang X,Hirano E

Tue Jun 01 00:00:00 UTC 2021

1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway.

Biochemical pharmacology

Zhou Y,Fu X,Guan Y,Gong M,He K,Huang B

Sat Feb 01 00:00:00 UTC 2020

Zika Virus NS3 Mimics a Cellular 14-3-3-Binding Motif to Antagonize RIG-I- and MDA5-Mediated Innate Immunity.

Cell host & microbe

Riedl W,Acharya D,Lee JH,Liu G,Serman T,Chiang C,Chan YK,Diamond MS,Gack MU

Wed Oct 09 00:00:00 UTC 2019

14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.

Journal of experimental & clinical cancer research : CR

Song J,Zhang X,Liao Z,Liang H,Chu L,Dong W,Zhang X,Ge Q,Liu Q,Fan P,Zhang Z,Zhang B

Thu Jan 03 00:00:00 UTC 2019

14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.

Journal of experimental & clinical cancer research : CR

Song J,Zhang X,Liao Z,Liang H,Chu L,Dong W,Zhang X,Ge Q,Liu Q,Fan P,Zhang Z,Zhang B

Thu Jan 03 00:00:00 UTC 2019

CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer.

BMC cancer

Li H,Jin X,Liu B,Zhang P,Chen W,Li Q

Thu Aug 22 00:00:00 UTC 2019

Interactome of the Autoimmune Risk Protein ANKRD55.

Frontiers in immunology

Ugidos N,Mena J,Baquero S,Alloza I,Azkargorta M,Elortza F,Vandenbroeck K

Thu Oct 29 00:00:00 UTC 2020

Interactome of the Autoimmune Risk Protein ANKRD55.

Frontiers in immunology

Ugidos N,Mena J,Baquero S,Alloza I,Azkargorta M,Elortza F,Vandenbroeck K

Thu Oct 29 00:00:00 UTC 2020

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Oncology letters

Lu K,Rui G,Liu F,Yang L,Deng X,Shi S,Li Q

Mon Jan 01 00:00:00 UTC 2018

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Oncology letters

Lu K,Rui G,Liu F,Yang L,Deng X,Shi S,Li Q

Mon Jan 01 00:00:00 UTC 2018

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Oncology letters

Lu K,Rui G,Liu F,Yang L,Deng X,Shi S,Li Q

Mon Jan 01 00:00:00 UTC 2018

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Oncology letters

Lu K,Rui G,Liu F,Yang L,Deng X,Shi S,Li Q

Mon Jan 01 00:00:00 UTC 2018

Specific Alterations in Astrocyte Properties via the GluA2-GAPDH Complex Associated with Multiple Sclerosis.

Scientific reports

Lee FHF,Zhang H,Jiang A,Zai CC,Liu F

Mon Aug 27 00:00:00 UTC 2018

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.

Nature immunology

Chan YK,Gack MU

Sun May 01 00:00:00 UTC 2016

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.

Nature immunology

Chan YK,Gack MU

Sun May 01 00:00:00 UTC 2016

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.

Nature immunology

Chan YK,Gack MU

Sun May 01 00:00:00 UTC 2016

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.

Nature immunology

Chan YK,Gack MU

Sun May 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: 14-3-3 epsilon; 14-3-3 protein epsilon; 14-3-3E; epididymis luminal protein 2; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; MSF L; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide

View more View less

Gene Aliases: 14-3-3E; AU019196; HEL2; KCIP-1; MDCR; MDS; YWHAE

View more View less

UniProt ID: (Human) P62258, (Rat) P62260, (Mouse) P62259

View more View less

Entrez Gene ID: (Human) 7531, (Rat) 29753, (Mouse) 22627

View more View less

Function(s)

protein binding potassium channel regulator activity enzyme binding protein domain specific binding MHC class II protein complex binding ubiquitin protein ligase binding histone deacetylase binding ion channel binding poly(A) RNA binding protein heterodimerization activity phosphoserine binding phosphoprotein binding cadherin binding involved in cell-cell adhesion protein kinase C inhibitor activity protein complex binding

Process(es)

neuron migration protein targeting signal transduction substantia nigra development hippocampus development cerebral cortex development negative regulation of protein dephosphorylation regulation of membrane repolarization negative regulation of protein serine/threonine kinase activity regulation of potassium ion transmembrane transporter activity negative regulation of peptidyl-serine dephosphorylation G2/M transition of mitotic cell cycle regulation of heart rate by hormone viral process hippo signaling intracellular signal transduction negative regulation of cysteine-type endopeptidase activity involved in apoptotic process membrane organization membrane repolarization during cardiac muscle cell action potential regulation of heart rate by cardiac conduction cell-cell adhesion regulation of cellular response to heat positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

14-3-3 epsilon Polyclonal Antibody

14-3-3 epsilon Antibody (11648-2-AP) in ICC/IF

Product Details

11648-2-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!