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14-3-3 epsilon Antibody (711273) in WB
Western blot analysis was performed on Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2) and Membrane extract of Jurkat (Lane 3), PC-12 (Lane 4), SW480 (Lane 5), NIH/3T3 (Lane 6), Hep G2 (Lane 7), HeLa (Lane 8) and T-47D (Lane 9). The blots were probed with Anti-14-3-3 protein epsilon/YWHAE Recombinant Rabbit Superclonal™ Antibody (Product # 711273, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500... View More
Product Details
711273
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
5HCLC
Immunogen
Peptides corresponding to Human YWHAE (aa 70-87, 238-255)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633146
Product Specific Information
This antibody is predicted to react with Monkey, Sheep, Rat and Mouse
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
14-3-3 epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3 epsilon; 14-3-3 protein epsilon; 14-3-3E; epididymis luminal protein 2; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; MSF L; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatioprotein epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
Gene Aliases: 14-3-3E; HEL2; KCIP-1; MDCR; MDS; YWHAE
UniProt ID: (Human) P62258, (Rat) P62260
Entrez Gene ID: (Human) 7531, (Rat) 29753
protein binding
potassium channel regulator activity
enzyme binding
protein domain specific binding
MHC class II protein complex binding
ubiquitin protein ligase binding
histone deacetylase binding
ion channel binding
poly(A) RNA binding
protein heterodimerization activity
phosphoserine binding
phosphoprotein binding
cadherin binding involved in cell-cell adhesion
protein kinase C inhibitor activity
protein complex binding
neuron migration
protein targeting
signal transduction
substantia nigra development
hippocampus development
cerebral cortex development
negative regulation of protein dephosphorylation
regulation of membrane repolarization
negative regulation of protein serine/threonine kinase activity
regulation of potassium ion transmembrane transporter activity
negative regulation of peptidyl-serine dephosphorylation
G2/M transition of mitotic cell cycle
regulation of heart rate by hormone
viral process
hippo signaling
intracellular signal transduction
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
membrane organization
membrane repolarization during cardiac muscle cell action potential
regulation of heart rate by cardiac conduction
cell-cell adhesion
regulation of cellular response to heat
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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