Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
5-Hydroxytryptophan Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
5-Hydroxytryptophan Antibody (PA5-119514) in ELISA
ELISA of 5-Hydroxytryptophan in 100 µL (coated at a concentration of 10 µg/mL) of 5-Hydroxytryptophan carrier protein conjugate. Detection range is 630 ng/mL down to 2.3 ng/mL (coated ligand detection range). Sample was incubated with polyclonal 5-Hydroxytryptophan antibody (Product # PA5-119514), followed by Rabbit IgG (HRP) with a dilution of 1:2000.
Product Details
PA5-119514
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
5-Hydroxytryptophan.
Conjugate
Form
Liquid
Concentration
0.63 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913088
Product Specific Information
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target Information
5-Hydroxytryptophan (5-HTP) is an intermediate metabolite in the biochemical synthesis of serotonin from the essential amino acid tryptophan. The conversion of tryptophan to 5-HTP involves the enzyme tryptophan hydroxylase (TPH), which exists in two isoforms: TPH1 and TPH2. TPH1 is mainly expressed in the peripheral tissues, while TPH2 is predominantly found in the central nervous system. Once synthesized, 5-HTP is further decarboxylated by aromatic L-amino acid decarboxylase (AADC) to produce serotonin (5-hydroxytryptamine, 5-HT). This biogenic amine plays a crucial role in regulating mood, cognition, and various physiological processes. Serotonin can also be acetylated and methylated to produce melatonin, a hormone involved in regulating sleep-wake cycles. Given its central role in serotonin biosynthesis, 5-HTP is often used in the treatment of conditions like depression, anxiety, and insomnia. Recent biotechnological advances have focused on optimizing the production of 5-HTP through engineered enzyme systems to meet increasing demand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support