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5-Methylcytosine (5-mC) Monoclonal Antibody (A1)
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5-Methylcytosine (5-mC) Antibody (61479) in MeDIP
5-Methylcytosine (5-mC) antibody (mAb) tested by Methyl-DNA Immunoprecipitation (MeDIP). Methylated DNA derived from the promoter of the APC gene was amplified using 5me-dCTP and PCR. 25pg of methylated DNA was spiked into 500 ng of MseI digested human genomic DNA and subjected to the MeDIP procedure using 5μg of 5-Methylcytidine antibody or control mouse IgG. Real time quantitative PCR to amplify the methy... View More
Product Details
61479
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
A1
Immunogen
This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to kLH and recognizes 5-Methylcytosine.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.5, with 30% glycerol
Contains
0.035% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2793653
Target Information
Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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