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Alomone Labs, LTD
5HT1B Receptor (HTR1B) (extracellular) Polyclonal Antibody, Atto™ 488
FIGURE: 1 / 1
5HT1B Receptor (HTR1B) (extracellular) Antibody (ASR-022-AG-50UL) in IHC
Expression of 5-Hydroxytryptamine receptor 1B in rat and mouse brain - Immunohistochemical staining of rat and mouse brain sections using Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody (#ASR-022-AG), (1:80). A. 5-HT1B receptor staining (green) in rat hippocampal CA1 region is detected near the pyramidal layer (P) and in apical dendrites (arrows). B. In mouse hippocampal CA1 region, 5-HT... View More
Product Details
ASR-022-AG-50UL
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CSAKDYIYQDSISLPWK, corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine receptor 1B, Extracellular, N-terminus
Conjugate
Excitation/Emission Max
501/523 nm
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. Molecular cloning studies have identified 5 receptors belonging to the 5-HT1 subtypes. These receptors are negatively coupled to adenylyl cyclase. Radioligand binding studies suggest the presence of 5-HT1B (formerly known as 5-HT1DbR) sites in substantia nigra, dorsal subiculum, globus pallidus, and superior collicum. In situ hybridization studies show abundance of 5-HT1BR mRNA in CA1 pyramidal cells of hippocampus, Purkinje cells of cerebellum, olfactory tubercle, subthalamic nucleus and somewhat lower levels in the caudate-putamen, cortical areas, thalamic and hypothalamic nuclei.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-HT-1B; 5-HT-1D-beta; 5-HT1B receptor; 5-HT1B serotonin receptor; 5-HT<1B> receptor; 5-hydroxytryptamine (serotonin ) receptor 1B; 5-hydroxytryptamine (serotonin) receptor 1B, G protein-coupled; 5-hydroxytryptamine receptor 1B; 5HT1B Receptor; S12; serotonin (5-hydroxytryptamine (5HT)) receptor type 1B; Serotonin 1D beta receptor; Serotonin receptor 1B
Gene Aliases: 5-HT-1B; 5-HT-1D-beta; 5-HT1B; 5-HT1DB; 5ht1b; HTR1B; HTR1D2; HTR1DB; S12
UniProt ID: (Human) P28222, (Mouse) P28334, (Rat) P28564
Entrez Gene ID: (Human) 3351, (Mouse) 15551, (Rat) 25075
G-protein coupled receptor internalization
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting serotonin receptor signaling pathway
protein kinase C-activating G-protein coupled receptor signaling pathway
serotonin receptor signaling pathway
chemical synaptic transmission
behavior
feeding behavior
response to toxic substance
regulation of dopamine secretion
negative regulation of serotonin secretion
negative regulation of cAMP biosynthetic process
negative regulation of synaptic transmission, GABAergic
cellular response to drug
response to cocaine
vasoconstriction
response to drug
drinking behavior
response to ethanol
bone remodeling
regulation of behavior
negative regulation of synaptic transmission, glutamatergic
cellular response to alkaloid
cellular response to temperature stimulus
response to mineralocorticoid
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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