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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • 6x His Tag Antibodies

          Invitrogen

          6x-His Tag Monoclonal Antibody (3D5)

          Advanced Verification
          13 Published Figures
          39 References
          View all (128) 6x His Tag antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite 6x-His Tag Monoclonal Antibody (3D5)

          • Antibody Testing Data (2)
          • Published Figures (13)
          • Advanced Verification (1)
          6x-His Tag Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          6x-His Tag Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 16

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          6x-His Tag Antibody (R930-25) in ICC/IF

          Immunofluorescent analysis of Histidine tag (6XHis) was done on HEK-293 cells transiently overexpressing His- IGFBP6. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Histidine (C-term) tag (6XHis) Mouse Monoclonal Antibody (Product # R930-25) at a dilution ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          6x-His Tag Antibody in Immunocytochemistry (ICC/IF)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Immunohistochemistry (IHC)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody in Western Blot (WB)
          6x-His Tag Antibody

          Product Details

          R930-25

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:5,000
          View 24 publications 24 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          View 5 publications 5 publications

          ELISA (ELISA)

          Assay-dependent
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          Assay-dependent
          View 1 publication 1 publication

          Luminex (LUM)

          -
          View 1 publication 1 publication

          In vitro Assay (IV)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 4 publications 4 publications
          Product Specifications

          Species Reactivity

          Tag

          Published species

          Tag

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3D5

          Immunogen

          6x His synthetic peptide

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • AP
          • FITC
          • HRP
          • Request custom conjugation

          Form

          Liquid

          Purification

          purified

          Storage buffer

          PBS

          Contains

          0.01% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          RRID

          AB_2556553

          Product Specific Information

          NOTE: R930-25 was previously sold under product #46-0693. When re-ordering, please use #R930-25.

          This antibody can be used to detect expression of C-terminal His fusion proteins from bacterial, insect, and mammalian cells.

          This product contains enough material for 25 Western blots. It is functionally tested using 500 ng of an E. coli expressed fusion protein containing a C-terminal polyhistidine (6xHis) epitope. A signal can be detected when tested against 100 ng of recombinant Positope™ protein. For Western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM).

          This product has also been used successfully to immunoprecipitate fusion proteins that contain the 6x His tag.

          Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.

          Target Information

          The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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