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AANAT Antibody (PA1-760) in IHC
Molecular pain 2021 -
Figure 5. Repeated-dose glibenclamide, administered either prophylactically or therapeutically, impedes or reverses the development of pain behaviors after PNI. (a) and (b) WT mice underwent sciatic n. cuffing on pod-0 and were tested for mechanical sensitivity using von Frey filaments (a) and for thermal sensitivity using the automated Hargreaves test (b) on the days denoted on the abscissas; the mice rece... View More
Product Details
PA1-760
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C(24) Q R R H T L P A S(33)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_325870
Product Specific Information
PA1-760 detects serotonin N-acetyltransferase (AA-NAT) from rat tissues.
PA1-760 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract.
PA1-760 immunizing phosphopeptide (Cat. # PEP-064) corresponds to amino-terminal amino acid residues 24-33 of mouse AA-NAT. Total anti-amino-terminal AA-NAT-specific IgG (Cat. # PA1-760) was affinity purified on resin-coupled non-phosphopeptide (Cat. # PEP-063). Phospho-specific amino-terminal IgG (Cat. # PA1-761) was subsequently isolated on resin-coupled phosphopeptide (Cat. # PEP-064). This sequence is completely conserved between mouse, rat, and human AA-NAT protein.
Target Information
Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatonin synthesis. Hormonal melatonin has been implicated in sleep and circadian clock function. Large changes in the abundance of AANAT are believed to be responsible for the large day/night rhythms in melatonin synthesis in the pineal gland and retina. Most research shows that AANAT activity is regulated by controlling the steady-state levels of the protein. Recent research, however, suggests that another mechanism may also play a role in controlling melatonin production without altering AANAT protein and activity. This mechanism may explain the sudden physiological increase in circulating melatonin observed at the onset of the dark period in humans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AA-NAT; Aralkylamine N-acetyltransferase; arylakylamine N-acetyltransferase; Arylalkylamine N - acetyltransferase (Serotonin N - acetyltransferase); arylalkylamine N-acetyltransferase; Seretonin N-acetyltransferase; Serotonin acetylase; Serotonin N-acetyltransferase
Gene Aliases: AA-NAT; Aanat; Nat4; Snat
UniProt ID: (Rat) Q64666
Entrez Gene ID: (Rat) 25120
N-terminal protein amino acid acetylation
circadian rhythm
response to light stimulus
photoperiodism
response to zinc ion
response to organic cyclic compound
melatonin biosynthetic process
response to insulin
response to cytokine
response to prostaglandin E
entrainment of circadian clock by photoperiod
response to copper ion
response to corticosterone
response to cAMP
response to calcium ion
cellular response to cAMP
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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