Invitrogen

ABCA1 Polyclonal Antibody

ABCA1 Antibody in Immunocytochemistry (ICC/IF)

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ABCA1 Antibody (PA1-16789) in ICC/IF

Immunofluorescence analysis of ABCA1 was performed using log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ABCA1 Polyclonal Antibody (3A1.891.3) (Product # PA1-16789) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilu... View More

ABCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ABCA1 Antibody in Immunoprecipitation (IP)

ABCA1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ABCA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of ABCA1 was observed in THP-1 differentiated to macrophages when compared to THP-1 using Anti-ABCA1 Polyclonal Antibody (Product # PA1-16789) in Western Blot. {RE}

Product Details

PA1-16789

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

1:400

ELISA (ELISA)

Assay-Dependent

Immunoprecipitation (IP)

1:10-1:500

ChIP assay (ChIP)

Assay-Dependent

Neutralization (Neu)

Assay-Dependent

Gel Shift (GS)

Assay-Dependent

Product Specifications

Species Reactivity

Dog, Chicken, Hamster, Human, Mustelid, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Partial peptide sequence corresponding to a sequence between residues 1100-1300 of human ABCA1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2288917

Product Specific Information

ABCA1 is known to aggregate.

DO NOT BOIL SAMPLES.

Target Information

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting Cholesterol Homeostasis Improves Recovery in Experimental Optic Neuritis.

Biomolecules

Godwin CR,Anders JJ,Cheng L,Elwood BW,Kardon RH,Gramlich OW

Published figure using ABCA1 polyclonal antibody (Product # PA1-16789) in Immunohistochemistry

Fri Oct 07 00:00:00 UTC 2022

Givinostat-Liposomes: Anti-Tumor Effect on 2D and 3D Glioblastoma Models and Pharmacokinetics.

Cancers

Taiarol L,Bigogno C,Sesana S,Kravicz M,Viale F,Pozzi E,Monza L,Carozzi VA,Meregalli C,Valtorta S,Moresco RM,Koch M,Barbugian F,Russo L,Dondio G,Steinkühler C,Re F

PA1-16789 was used in Western Blotting to provide the proof of concept for a delivery system that can improve the in vivo half-life and increase the brain delivery of Givinostat, a pan-HDAC inhibitor.

Thu Jun 16 00:00:00 UTC 2022

Liver RBFOX2 regulates cholesterol homeostasis via Scarb1 alternative splicing in mice.

Nature metabolism

Paterson HAB,Yu S,Artigas N,Prado MA,Haberman N,Wang YF,Jobbins AM,Pahita E,Mokochinski J,Hall Z,Guerin M,Paulo JA,Ng SS,Villarroya F,Rashid ST,Le Goff W,Lenhard B,Cebola I,Finley D,Gygi SP,Sibley CR,Vernia S

PA1-16789 was used in ChIP Assay, ELISA, Flow Cytometry, Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Western Blot to identify a key role for the splicing factor RNA-binding Fox protein 2 (RBFOX2) in maintaining cholesterol homeostasis in a lipogenic environment in the liver.

Thu Dec 01 00:00:00 UTC 2022

CTRP12 ameliorates atherosclerosis by promoting cholesterol efflux and inhibiting inflammatory response via the miR-155-5p/LXRα pathway.

Cell death & disease

Wang G,Chen JJ,Deng WY,Ren K,Yin SH,Yu XH

PA1-16789 was used in Western Blot to suggest that CTRP12 protects against atherosclerosis by enhancing RCT efficiency and mitigating vascular inflammation via the miR-155-5p/LXRα pathway.

Wed Mar 10 00:00:00 UTC 2021

CTRP12 ameliorates atherosclerosis by promoting cholesterol efflux and inhibiting inflammatory response via the miR-155-5p/LXRα pathway.

Cell death & disease

Wang G,Chen JJ,Deng WY,Ren K,Yin SH,Yu XH

PA1-16789 was used in Western Blot to suggest that CTRP12 protects against atherosclerosis by enhancing RCT efficiency and mitigating vascular inflammation via the miR-155-5p/LXRα pathway.

Wed Mar 10 00:00:00 UTC 2021

Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytes.

Osteoarthritis and cartilage open

Sun MM,Beier F

PA1-16789 was used in ChIP Assay, ELISA, Flow Cytometry, Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Western Blot to show that LXR activation alters expression of lipid metabolism genes in growth plate chondrocytes, in part through activation of molecules responsible for cellular cholesterol efflux.

Sun Mar 01 00:00:00 UTC 2020

Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytes.

Osteoarthritis and cartilage open

Sun MM,Beier F

PA1-16789 was used in ChIP Assay, ELISA, Flow Cytometry, Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Western Blot to show that LXR activation alters expression of lipid metabolism genes in growth plate chondrocytes, in part through activation of molecules responsible for cellular cholesterol efflux.

Sun Mar 01 00:00:00 UTC 2020

PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol Efflux.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Zhou Y,Miles JR,Tavori H,Lin M,Khoshbouei H,Borchelt DR,Bazick H,Landreth GE,Lee S,Fazio S,Notterpek L

PA1-16789 was used in Immunocytochemistry-immunoflourescence to reveal a novel role for PMP22 in regulating lipid metabolism and cholesterol trafficking through functional interaction with the cholesterol efflux regulatory protein, ABCA1.

Wed Jul 03 00:00:00 UTC 2019

PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol Efflux.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Zhou Y,Miles JR,Tavori H,Lin M,Khoshbouei H,Borchelt DR,Bazick H,Landreth GE,Lee S,Fazio S,Notterpek L

Wed Jul 03 00:00:00 UTC 2019

The Liver X Receptor Is Upregulated in Monocyte-Derived Macrophages and Modulates Inflammatory Cytokines Based on LXRα Polymorphism.

Mediators of inflammation

Kim HA,Baek WY,Han MH,Jung JY,Suh CH

PA1-16789 was used in Western Blotting to suggest that the expression levels of LXRα, according to LXRα -1830 T > C genotype, may contribute to the inflammatory response by induction of inflammatory cytokines in SLE.

Mon Aug 05 00:00:00 UTC 2019

ATP Binding Cassette Transporter A1 is Involved in Extracellular Secretion of Acetylated APE1/Ref-1.

International journal of molecular sciences

Lee YR,Joo HK,Lee EO,Cho HS,Choi S,Kim CS,Jeon BH

PA1-16789 was used in Western blotting to show acetylation of APE1/Ref-1 is considered to be necessary for its extracellular targeting via non-classical secretory pathway using the ABCA1 transporter.

Fri Jun 28 00:00:00 UTC 2019

Calpains Released by T Lymphocytes Cleave TLR2 To Control IL-17 Expression.

Journal of immunology (Baltimore, Md. : 1950)

Perez J,Dansou B,Hervé R,Levi C,Tamouza H,Vandermeersch S,Demey-Thomas E,Haymann JP,Zafrani L,Klatzmann D,Boissier MC,Letavernier E,Baud L

PA1-16789 was used in western blot to elucidate the role of calpains from T lymphocytes and IL-17 expression

Fri Jan 01 00:00:00 UTC 2016

Xanthine oxidoreductase is involved in macrophage foam cell formation and atherosclerosis development.

Arteriosclerosis, thrombosis, and vascular biology

Kushiyama A,Okubo H,Sakoda H,Kikuchi T,Fujishiro M,Sato H,Kushiyama S,Iwashita M,Nishimura F,Fukushima T,Nakatsu Y,Kamata H,Kawazu S,Higashi Y,Kurihara H,Asano T

PA1-16789 was used in western blot to study the role of xanthine oxidoreductase in macrophage foam cell formation and the effect of a xanthine oxidireductase inhibitor on atherosclerosis

Wed Feb 01 00:00:00 UTC 2012

Binding of PDZ-RhoGEF to ATP-binding cassette transporter A1 (ABCA1) induces cholesterol efflux through RhoA activation and prevention of transporter degradation.

The Journal of biological chemistry

Okuhira K,Fitzgerald ML,Tamehiro N,Ohoka N,Suzuki K,Sawada J,Naito M,Nishimaki-Mogami T

PA1-16789 was used in western blot to investigate the mechanism of cholesterol efflux mediated by ABCA1-PDZ-RhoGEF interaction

Fri May 21 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: ABC 1; ABC 1 antibody; ABC Transporter 1; ABC-1; ABC1 antibody; ATP-binding cassette 1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ATP-binding cassette transporter A1; ATP-binding cassette, sub-family A (ABC1), member 1; CERP antibody; Cholesterol efflux regulatory protein; membrane-bound; Phospholipid-transporting ATPase ABCA1

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Gene Aliases: ABC-1; ABC1; ABCA1; CERP; HDLDT1; TGD

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UniProt ID: (Human) Q96T85, (Mouse) P41233

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Entrez Gene ID: (Chicken) 373945, (Dog) 481651, (Rabbit) 100356765, (Human) 19, (Mouse) 11303, (Rat) 313210, (Chinese hamster) 100761226

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Function(s)

receptor binding protein binding ATP binding phospholipid binding phospholipid transporter activity anion transmembrane transporter activity cholesterol binding cholesterol transporter activity syntaxin binding small GTPase binding apolipoprotein binding apolipoprotein A-I binding apolipoprotein A-I receptor activity ATPase activity, coupled to transmembrane movement of substances ATPase binding nucleotide binding transporter activity ATPase activity

Process(es)

peptide secretion protein lipidation transport phagocytosis, engulfment lysosome organization G-protein coupled receptor signaling pathway cholesterol metabolic process positive regulation of cholesterol efflux phospholipid transport endosomal transport cholesterol transport positive regulation of cAMP biosynthetic process intracellular cholesterol transport regulation of Cdc42 protein signal transduction cholesterol efflux phospholipid efflux high-density lipoprotein particle assembly apolipoprotein A-I-mediated signaling pathway lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis reverse cholesterol transport phospholipid translocation interleukin-1 beta secretion transmembrane transport phospholipid homeostasis response to high density lipoprotein particle platelet dense granule organization cellular response to lipopolysaccharide cellular response to retinoic acid anion transmembrane transport response to nutrient ion transmembrane transport response to laminar fluid shear stress response to drug response to low-density lipoprotein particle cellular response to cholesterol negative regulation of macrophage derived foam cell differentiation negative regulation of cholesterol storage

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ABCA1 Polyclonal Antibody

ABCA1 Antibody (PA1-16789) in ICC/IF

Product Details

PA1-16789

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Neutralization (Neu)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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