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Invitrogen
ABCA1 Recombinant Rabbit Monoclonal Antibody (10G8Z7)
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FIGURE: 1 / 3
ABCA1 Antibody (MA5-55376) in ICC/IF
Immunocytochemistry analysis of Hep G2 cells using ABCA1 Recombinant Monoclonal Antibody (Product # MA5-55376) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red). The cells were counterstained with alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Product Details
MA5-55376
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10G8Z7
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1170-1350 of human ABCA1.
Conjugate
Form
Liquid
Concentration
1.04 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667617
Product Specific Information
Immunogen Sequence: DHESDTLTID VSAISNLIRK HVSEARLVED IGHELTYVLP YEAAKEGAFV ELFHEIDDRL SDLGISSYGI SETTLEEIFL KVAEESGVDA ETSDGTLPAR RNRRAFGDKQ SCLRPFTEDD AADPNDSDID PESRETDLLS GMDGKGSYQV KGWKLTQQQF VALLWKRLLI ARRSRKGFFA Q
Target Information
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ABC 1; ABC 1 antibody; ABC Transporter 1; ABC-1; ABC1 antibody; ATP-binding cassette 1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ATP-binding cassette transporter A1; ATP-binding cassette, sub-family A (ABC1), member 1; CERP antibody; Cholesterol efflux regulatory protein; membrane-bound; Phospholipid-transporting ATPase ABCA1
Gene Aliases: ABC-1; ABC1; ABCA1; CERP; HDLDT1; TGD
UniProt ID: (Human) Q96T85, (Mouse) P41233
Entrez Gene ID: (Human) 19, (Mouse) 11303, (Rat) 313210
receptor binding
protein binding
ATP binding
phospholipid binding
phospholipid transporter activity
anion transmembrane transporter activity
cholesterol binding
cholesterol transporter activity
syntaxin binding
small GTPase binding
apolipoprotein binding
apolipoprotein A-I binding
apolipoprotein A-I receptor activity
ATPase activity, coupled to transmembrane movement of substances
ATPase binding
nucleotide binding
transporter activity
ATPase activity
peptide secretion
protein lipidation
transport
phagocytosis, engulfment
lysosome organization
G-protein coupled receptor signaling pathway
cholesterol metabolic process
positive regulation of cholesterol efflux
phospholipid transport
endosomal transport
cholesterol transport
positive regulation of cAMP biosynthetic process
intracellular cholesterol transport
regulation of Cdc42 protein signal transduction
cholesterol efflux
phospholipid efflux
high-density lipoprotein particle assembly
apolipoprotein A-I-mediated signaling pathway
lipoprotein metabolic process
lipoprotein biosynthetic process
cholesterol homeostasis
reverse cholesterol transport
phospholipid translocation
interleukin-1 beta secretion
transmembrane transport
phospholipid homeostasis
response to high density lipoprotein particle
platelet dense granule organization
cellular response to lipopolysaccharide
cellular response to retinoic acid
anion transmembrane transport
response to nutrient
ion transmembrane transport
response to laminar fluid shear stress
response to drug
response to low-density lipoprotein particle
cellular response to cholesterol
negative regulation of macrophage derived foam cell differentiation
negative regulation of cholesterol storage
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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