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ABRA Antibody (PA5-144020) in WB
Western blot analysis of ABRA using ABRA antibody (Product # PA5-144020). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: rat heart tissue lysates; Lane 2: mouse heart tissue lysates; Lane 3: mouse skeletal muscle tissue lysates; Lane 4: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked... View More
Product Details
PA5-144020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ABRA recombinant protein (Position: A13-K381).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075234
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: rat heart tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse C2C12 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Acts as an activator of serum response factor (SRF)-dependent transcription possibly by inducing nuclear translocation of MKL1 or MKL2 and through a mechanism requiring Rho-actin signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ABRA; abra {ECO:0000312|RGD:708493}; actin-binding Rho activating protein; Actin-binding Rho-activating protein; MS1; STARS; Striated muscle activator of Rho-dependent signaling
Gene Aliases: ABRA; C130068O12Rik; Ms1; STARS
UniProt ID: (Human) Q8N0Z2, (Mouse) Q8BUZ1, (Rat) Q8K4K7
Entrez Gene ID: (Human) 137735, (Mouse) 223513, (Rat) 286965
protein import into nucleus, translocation
transcription, DNA-templated
regulation of transcription, DNA-templated
transport
protein transport
positive regulation of Rho protein signal transduction
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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