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Invitrogen
ACAD9 Polyclonal Antibody
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FIGURE: 1 / 1
ACAD9 Antibody (PA5-121139) in WB
Western blot analysis of ACAD9 in extracts of various cell lines. Samples were incubated with ACAD9 Polyclonal antibody (Product # PA5-121139) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-121139
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ACAD9 (NP_054768.2).
Conjugate
Form
Liquid
Concentration
0.88 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914711
Product Specific Information
Positive test controls include: SKOV3, U-251MG, MCF7, 293T, NCI-H460, Mouse kidney, Mouse liver, Mouse brain. The target is usually found in the following locations: Mitochondrion.
Immunogen sequence: MSGCGLFLRT TAAARACRGL VVSTANRRLL RTSPPVRAFA KELFLGKIKK KEVFPFPEVS QDELNEINQF LGPVEKFFTE EVDSRKIDQE GKIPDETLEK LKSLGLFGLQ VPEEYGGLGF SNTMYSRLGE IISMDGSITV TLAAHQAIGL KGIILAGTEE QKAKYLPKLA SGEHIAAFCL TEPASGSDAA SIRSRATLSE DKKHYILNGS KVWITNGGLA NIFTVFAKTE VVDSDGSVKD KITAFIVERD FGGVTNGKPE DKLGIRGSNT
Target Information
This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACAD-9; Acyl-CoA dehydrogenase family member 9; acyl-CoA dehydrogenase family member 9, mitochondrial; acyl-CoA dehydrogenase family, member 9; acyl-Coenzyme A dehydrogenase family, member 9; Complex I assembly factor ACAD9, mitochondrial; very-long-chain acyl-CoA dehydrogenase VLCAD; very-long-chain acyl-CoA dehydrogenase VLCAD homolog
Gene Aliases: 2600017P15Rik; 4732402K02; ACAD9; C630012L17Rik; NPD002
UniProt ID: (Human) Q9H845, (Mouse) Q8JZN5
Entrez Gene ID: (Human) 28976, (Mouse) 229211
fatty-acyl-CoA binding
acyl-CoA dehydrogenase activity
protein binding
electron carrier activity
flavin adenine dinucleotide binding
oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
short-branched-chain-acyl-CoA dehydrogenase activity
very-long-chain-acyl-CoA dehydrogenase activity
4-methyloctanoyl-CoA dehydrogenase activity
naphthyl-2-methyl-succinyl-CoA dehydrogenase activity
2-methylhexanoyl-CoA dehydrogenase activity
propionyl-CoA dehydrogenase activity
thiol-driven fumarate reductase activity
coenzyme F420-dependent 2,4,6-trinitrophenol reductase activity
coenzyme F420-dependent 2,4,6-trinitrophenol hydride reductase activity
coenzyme F420-dependent 2,4-dinitrophenol reductase activity
medium-chain-acyl-CoA dehydrogenase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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