Invitrogen

ACE2 Recombinant Rabbit Monoclonal Antibody (SN0754)

ACE2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ACE2 Antibody (MA5-32307) in IHC (P)

Immunohistochemical analysis of ACE2 of paraffin-embedded Human breast carcinoma tissue using a ACE2 Monoclonal antibody (Product #MA5-32307). Counter stained with hematoxylin.

ACE2 Antibody in Immunocytochemistry (ICC/IF)

ACE2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

ACE2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ACE2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across all cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of Angiotensin-converting enzyme 2 was observed in HaCaT, Hep G2 , RT4, Mouse Testis and Rat Testis but not in BJ, U-2 OS, Mouse Skeletal Muscle and Rat Skeletal Muscle which are known to have comparatively lower expression (DOI: 10.3892/mmr.2014.2266, 10.1038/s41433-020-0939-4) using Anti-ACE2 Recombinant Rabbit Monoclonal Antibody (SN0754) (Product # MA5-32307) in Western Blot. {RE}

Product Details

MA5-32307

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:500

View 6 publications 6 publications

Flow Cytometry (Flow)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SN0754

Immunogen

Synthetic peptide within Human ACE2 aa 181-230

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2809589

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SARS-CoV-2 Spike protein suppresses CTL-mediated killing by inhibiting immune synapse assembly.

The Journal of experimental medicine

Onnis A,Andreano E,Cassioli C,Finetti F,Della Bella C,Staufer O,Pantano E,Abbiento V,Marotta G,D'Elios MM,Rappuoli R,Baldari CT

MA5-32307 was used in Western Blotting to highlight a new strategy of immune evasion by SARS-CoV-2 based on the Spike-dependent, ACE2-mediated targeting of the lytic IS to prevent elimination of infected cells.

Mon Feb 06 00:00:00 UTC 2023

SARS-CoV-2 Spike protein suppresses CTL-mediated killing by inhibiting immune synapse assembly.

The Journal of experimental medicine

Onnis A,Andreano E,Cassioli C,Finetti F,Della Bella C,Staufer O,Pantano E,Abbiento V,Marotta G,D'Elios MM,Rappuoli R,Baldari CT

Mon Feb 06 00:00:00 UTC 2023

Correlating the differences in the receptor binding domain of SARS-CoV-2 spike variants on their interactions with human ACE2 receptor.

Scientific reports

Mahalingam G,Arjunan P,Periyasami Y,Dhyani AK,Devaraju N,Rajendiran V,Christopher AC,Kt RD,Dhanasingh I,Thangavel S,Murugesan M,Moorthy M,Srivastava A,Marepally S

MA5-32307 was used in Western Blotting to provide a precise method to evaluate the binding interactions between spike sub-lineages to hACE2 receptors.

Tue May 30 00:00:00 UTC 2023

ACE-2, TMPRSS2, and Neuropilin-1 Receptor Expression on Human Brain Astrocytes and Pericytes and SARS-CoV-2 Infection Kinetics.

International journal of molecular sciences

Malik JR,Acharya A,Avedissian SN,Byrareddy SN,Fletcher CV,Podany AT,Dyavar SR

MA5-32307 was used in Immunocytochemistry to show that SARS-CoV-2 cellular entry receptor expression and in vitro" viral kinetics in astrocytes and pericytes."

Thu May 11 00:00:00 UTC 2023

Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs.

PLoS biology

Loo L,Waller MA,Moreno CL,Cole AJ,Stella AO,Pop OT,Jochum AK,Ali OH,Denes CE,Hamoudi Z,Chung F,Aggarwal A,Low JKK,Patel K,Siddiquee R,Kang T,Mathivanan S,Mackay JP,Jochum W,Flatz L,Hesselson D,Turville S,Neely GG

MA5-32307 was used in Immunocytochemistry, Immunohistochemistry, Western Blot, Flow Cytometry to conclude that LRRC15 is a novel SARS-CoV-2 spike-binding receptor that can help control viral load and regulate antiviral and antifibrotic transcriptional programs in the context of COVID-19 infection.

Wed Feb 01 00:00:00 UTC 2023

SARS-CoV-2 RBD and Its Variants Can Induce Platelet Activation and Clearance: Implications for Antibody Therapy and Vaccinations against COVID-19.

Research (Washington, D.C.)

Ma X,Liang J,Zhu G,Bhoria P,Shoara AA,MacKeigan DT,Khoury CJ,Slavkovic S,Lin L,Karakas D,Chen Z,Prifti V,Liu Z,Shen C,Li Y,Zhang C,Dou J,Rousseau Z,Zhang J,Ni T,Lei X,Chen P,Wu X,Shaykhalishahi H,Mubareka S,Connelly KA,Zhang H,Rotstein O,Ni H

MA5-32307 was used in Western Blotting to show that the RBD can bind platelets partially though αIIbβ3 and induce platelet activation and clearance, which may contribute to thrombosis and thrombocytopenia observed in COVID-19 and VITT.

Thu May 25 00:00:00 UTC 2023

COVID-19 mRNA vaccine protects against SARS-CoV-2 Omicron BA.1 infection in diet-induced obese mice through boosting host innate antiviral responses.

EBioMedicine

Chen Y,Song W,Li C,Wang J,Liu F,Ye Z,Ren P,Tong Y,Li J,Ou Z,Lee AC,Cai JP,Wong BH,Chan JF,Yuen KY,Zhang AJ,Chu H

MA5-32307 was used in Immunohistochemistry to suggest that COVID-19 mRNA vaccination enhances host innate antiviral responses in obesity which protect the DIO mice to a certain degree when adaptive immunity is suboptimal.

Wed Mar 01 00:00:00 UTC 2023

More tools for our toolkit: The application of HEL-299 cells and dsRNA-nanoparticles to study human coronaviruses in vitro.

Virus research

Semple SL,Alkie TN,Jenik K,Warner BM,Tailor N,Kobasa D,DeWitte-Orr SJ

Published figure using ACE2 recombinant monoclonal antibody (Product # MA5-32307) in Immunocytochemistry

Tue Nov 01 00:00:00 UTC 2022

Identification of a pharmacological approach to reduce ACE2 expression and development of an in vitro COVID-19 viral entry model.

Journal of virus eradication

Endo Y,Hickerson BT,Ilyushina NA,Mohan N,Peng H,Takeda K,Donnelly RP,Wu WJ

Published figure using ACE2 recombinant monoclonal antibody (Product # MA5-32307) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2022

SARS-CoV-2 down-regulates ACE2 through lysosomal degradation.

Molecular biology of the cell

Lu Y,Zhu Q,Fox DM,Gao C,Stanley SA,Luo K

MA5-32307 was used in Western Blotting to suggest that ACE2 down-regulation represents an important mechanism underlying SARS-CoV-2-associated pathology, and blocking this process could be a promising therapeutic strategy.

Thu Dec 01 00:00:00 UTC 2022

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.

Cell

Daniloski Z,Jordan TX,Wessels HH,Hoagland DA,Kasela S,Legut M,Maniatis S,Mimitou EP,Lu L,Geller E,Danziger O,Rosenberg BR,Phatnani H,Smibert P,Lappalainen T,tenOever BR,Sanjana NE

Thu Jan 07 00:00:00 UTC 2021

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.

Cell

Daniloski Z,Jordan TX,Wessels HH,Hoagland DA,Kasela S,Legut M,Maniatis S,Mimitou EP,Lu L,Geller E,Danziger O,Rosenberg BR,Phatnani H,Smibert P,Lappalainen T,tenOever BR,Sanjana NE

Thu Jan 07 00:00:00 UTC 2021

Selinexor, a novel selective inhibitor of nuclear export, reduces SARS-CoV-2 infection and protects the respiratory system in vivo.

Antiviral research

Kashyap T,Murray J,Walker CJ,Chang H,Tamir S,Hou B,Shacham S,Kauffman MG,Tripp RA,Landesman Y

MA5-32307 was used in Immunocytochemistry to provide in vitro and in vivo analysis of the efficacy of selinexor on the modulation of the anti-viral and the anti-inflammatory effects of selinexor in the context of SARS-CoV-2.

Sun Aug 01 00:00:00 UTC 2021

Selinexor, a novel selective inhibitor of nuclear export, reduces SARS-CoV-2 infection and protects the respiratory system in vivo.

Antiviral research

Kashyap T,Murray J,Walker CJ,Chang H,Tamir S,Hou B,Shacham S,Kauffman MG,Tripp RA,Landesman Y

Sun Aug 01 00:00:00 UTC 2021

Human Nasal and Lung Tissues Infected Ex Vivo with SARS-CoV-2 Provide Insights into Differential Tissue-Specific and Virus-Specific Innate Immune Responses in the Upper and Lower Respiratory Tract.

Journal of virology

Alfi O,Yakirevitch A,Wald O,Wandel O,Izhar U,Oiknine-Djian E,Nevo Y,Elgavish S,Dagan E,Madgar O,Feinmesser G,Pikarsky E,Bronstein M,Vorontsov O,Jonas W,Ives J,Walter J,Zakay-Rones Z,Oberbaum M,Panet A,Wolf DG

MA5-32307 was used in Immunohistochemistry to investigate viral susceptibility and early patterns of local mucosal innate immune response in the authentic milieu of the human respiratory tract. SARS-CoV-2 productively infected the nasal turbinate tissues, predominantly targeting respiratory epithelial cells, with a rapid increase in tissue-associated viral subgenomic mRNA and secretion of infectious viral progeny.

Thu Jun 24 00:00:00 UTC 2021

Dodging COVID-19 infection: low expression and localization of ACE2 and TMPRSS2 in multiple donor-derived lines of human umbilical cord-derived mesenchymal stem cells.

Journal of translational medicine

Hernandez JJ,Beaty DE,Fruhwirth LL,Lopes Chaves AP,Riordan NH

Published figure using ACE2 recombinant monoclonal antibody (Product # MA5-32307) in Flow Cytometry

Wed Apr 14 00:00:00 UTC 2021

Dodging COVID-19 infection: low expression and localization of ACE2 and TMPRSS2 in multiple donor-derived lines of human umbilical cord-derived mesenchymal stem cells.

Journal of translational medicine

Hernandez JJ,Beaty DE,Fruhwirth LL,Lopes Chaves AP,Riordan NH

Published figure using ACE2 recombinant monoclonal antibody (Product # MA5-32307) in Flow Cytometry

Wed Apr 14 00:00:00 UTC 2021

Dodging COVID-19 infection: low expression and localization of ACE2 and TMPRSS2 in multiple donor-derived lines of human umbilical cord-derived mesenchymal stem cells.

Journal of translational medicine

Hernandez JJ,Beaty DE,Fruhwirth LL,Lopes Chaves AP,Riordan NH

Published figure using ACE2 recombinant monoclonal antibody (Product # MA5-32307) in Flow Cytometry

Wed Apr 14 00:00:00 UTC 2021

Targeting Polyamines Inhibits Coronavirus Infection by Reducing Cellular Attachment and Entry.

ACS infectious diseases

Firpo MR,Mastrodomenico V,Hawkins GM,Prot M,Levillayer L,Gallagher T,Simon-Loriere E,Mounce BC

MA5-32307 was used in Immunocytochemistry to show coronavirus relies on polyamines for replication and thus could be a novel drug target.

Fri Jun 11 00:00:00 UTC 2021

Spike protein multiorgan tropism suppressed by antibodies targeting SARS-CoV-2.

Communications biology

Brady M,McQuaid C,Solorzano A,Johnson A,Combs A,Venkatraman C,Rahman A,Leyva H,Kwok WE,Wood RW,Deane R

MA5-32307 was used in Immunohistochemistry to show that SARS-CoV-2 spike protein had a body-wide biodistribution, slow regional elimination, except for the liver, which showed an accumulation, and differential organ uptake.

Mon Nov 22 00:00:00 UTC 2021

Lung cancer models reveal SARS-CoV-2-induced EMT contributes to COVID-19 pathophysiology.

bioRxiv : the preprint server for biology

Stewart CA,Gay CM,Ramkumar K,Cargill KR,Cardnell RJ,Nilsson MB,Heeke S,Park EM,Kundu ST,Diao L,Wang Q,Shen L,Xi Y,Zhang B,Della Corte CM,Fan Y,Kundu K,Gao B,Avila K,Pickering CR,Johnson FM,Zhang J,Kadara H,Minna JD,Gibbons DL,Wang J,Heymach JV,Byers LA

MA5-32307 was used in Western Blotting to investigate the expression and regulation of ACE2 and TMPRSS2, which are the mian co-factors for SARS-CoV-2 enter host cells.

Thu Jan 28 00:00:00 UTC 2021

Elevated ACE-2 expression in the olfactory neuroepithelium: implications for anosmia and upper respiratory SARS-CoV-2 entry and replication.

The European respiratory journal

Chen M,Shen W,Rowan NR,Kulaga H,Hillel A,Ramanathan M,Lane AP

MA5-32307 was used in Immunohistochemistry to determine the location of angiotensin-converting enzyme 2(ACE-2) in human nasal and tracheal specimens.

Tue Sep 01 00:00:00 UTC 2020

Elevated ACE-2 expression in the olfactory neuroepithelium: implications for anosmia and upper respiratory SARS-CoV-2 entry and replication.

The European respiratory journal

Chen M,Shen W,Rowan NR,Kulaga H,Hillel A,Ramanathan M,Lane AP

MA5-32307 was used in Immunohistochemistry to determine the location of angiotensin-converting enzyme 2(ACE-2) in human nasal and tracheal specimens.

Tue Sep 01 00:00:00 UTC 2020

Human Induced Pluripotent Stem Cell-Derived Lung Epithelial System for SARS-CoV-2 Infection Modeling and Its Potential in Drug Repurposing.

Stem cells and development

Surendran H,Nandakumar S,Pal R

MA5-32307 was used in Immunocytochemistry-immunoflourescence to show preliminary data on how human induced pluripotent stem cells-derived lung epithelial cell system could emerge as a relevant and sensitive platform for modeling SARS-CoV-2 infection and drug screening.

Sun Nov 01 00:00:00 UTC 2020

Tissue Distribution of ACE2 Protein in Syrian Golden Hamster (Mesocricetus auratus) and Its Possible Implications in SARS-CoV-2 Related Studies.

Frontiers in pharmacology

Suresh V,Parida D,Minz AP,Sethi M,Sahoo BS,Senapati S

Published figure using ACE2 recombinant monoclonal antibody (Product # MA5-32307) in Immunohistochemistry

Sat Mar 30 00:00:00 UTC 2024

Tissue Distribution of ACE2 Protein in Syrian Golden Hamster (Mesocricetus auratus) and Its Possible Implications in SARS-CoV-2 Related Studies.

Frontiers in pharmacology

Suresh V,Parida D,Minz AP,Sethi M,Sahoo BS,Senapati S

Published figure using ACE2 recombinant monoclonal antibody (Product # MA5-32307) in Immunohistochemistry

Sat Mar 30 00:00:00 UTC 2024

Tissue Distribution of ACE2 Protein in Syrian Golden Hamster (Mesocricetus auratus) and Its Possible Implications in SARS-CoV-2 Related Studies.

Frontiers in pharmacology

Suresh V,Parida D,Minz AP,Sethi M,Sahoo BS,Senapati S

Published figure using ACE2 recombinant monoclonal antibody (Product # MA5-32307) in Immunohistochemistry

Sat Mar 30 00:00:00 UTC 2024

Tissue Distribution of ACE2 Protein in Syrian Golden Hamster (Mesocricetus auratus) and Its Possible Implications in SARS-CoV-2 Related Studies.

Frontiers in pharmacology

Suresh V,Parida D,Minz AP,Sethi M,Sahoo BS,Senapati S

Published figure using ACE2 recombinant monoclonal antibody (Product # MA5-32307) in Immunohistochemistry

Sat Mar 30 00:00:00 UTC 2024

Bioinformatics

Protein Aliases: ACE 2; ACE-related carboxypeptidase; ACEH; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; Angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; Angiotensin-converting enzyme-related carboxypeptidase; anigotensin-converting enzyme-related carboxypeptidase; Metalloprotease MPROT15; peptidyl-dipeptidase A; renal angiotensin-converting enzyme 2

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Gene Aliases: 2010305L05Rik; ACE2; ACEH; UNQ868/PRO1885

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UniProt ID: (Human) Q9BYF1, (Rat) Q5EGZ1, (Mouse) Q8R0I0

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Entrez Gene ID: (Human) 59272, (Rat) 302668, (Mouse) 70008

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Function(s)

virus receptor activity glycoprotein binding endopeptidase activity carboxypeptidase activity metallocarboxypeptidase activity protein binding metallopeptidase activity peptidyl-dipeptidase activity zinc ion binding metal ion binding peptidase activity hydrolase activity

Process(es)

angiotensin-mediated drinking behavior regulation of systemic arterial blood pressure by renin-angiotensin proteolysis response to virus receptor biosynthetic process viral entry into host cell receptor-mediated virion attachment to host cell negative regulation of smooth muscle cell proliferation maternal process involved in female pregnancy negative regulation of ERK1 and ERK2 cascade positive regulation of gap junction assembly regulation of cardiac conduction regulation of cytokine production angiotensin maturation angiotensin catabolic process in blood tryptophan transport regulation of vasoconstriction regulation of cell proliferation regulation of vasodilation regulation of inflammatory response positive regulation of cardiac muscle contraction positive regulation of reactive oxygen species metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ACE2 Recombinant Rabbit Monoclonal Antibody (SN0754)

ACE2 Antibody (MA5-32307) in IHC (P)

Product Details

MA5-32307

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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