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Invitrogen
ACOT9 Polyclonal Antibody
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FIGURE: 1 / 3
ACOT9 Antibody (PA5-30476) in ICC/IF
ACOT9 Polyclonal Antibody detects ACOT9 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACOT9 protein stained by ACOT9 Polyclonal Antibody (Product # PA5-30476) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-30476
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ACOT9. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.84 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547950
Product Specific Information
Recommended positive controls: NT2D1, U87-MG.
Predicted reactivity: Mouse (81%), Rat (81%), Bovine (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of acyl-CoA to form coenzyme A (CoA) and a free fatty acid. Through their catalytic activity, ACOTs are able to regulate the level of fatty acids and acyl-CoAs within the cell. ACOT9 (acyl-CoA thioesterase 9), also known as ACATE2, MT-ACT48 (mitochondrial acyl-CoA thioesterase of 48 kDa) or CGI-16, is a 406 amino acid member of the acyl-CoA hydrolase protein family. ACOT9 contains a C-terminal 80-amino acid domain that is conserved from mouse to human, suggesting that the C-terminus may confer the catalytic activity of ACOT9. The gene encoding ACOT9 is located on chromosome X and the expressed ACOT9 protein is localized to the mitochondrion.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acyl-CoA thioester hydrolase 9; Acyl-CoA thioesterase 9; acyl-Coenzyme A thioesterase 2, mitochondrial; Acyl-coenzyme A thioesterase 9, mitochondrial; mitochondrial Acyl-CoA Thioesterase
Gene Aliases: ACATE2; ACOT9; CGI-16; MT-ACT48; MTACT48
UniProt ID: (Human) Q9Y305
Entrez Gene ID: (Human) 23597
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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