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Invitrogen
ACSL4 Monoclonal Antibody (4I7)
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FIGURE: 1 / 9
ACSL4 Antibody (MA5-49273) in ICC/IF
Immunocytochemistry analysis of ACSL4 in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in ACSL4 Monoclonal antibody (Product # MA5-49273) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
Product Details
MA5-49273
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4I7
Immunogen
A synthetic peptide corresponding to a sequence of human FACL4/ACSL4 (EPWTPETGLVTDAFKLKRKELR).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074931
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human PC-3 whole cell, human Hela whole cell, human Caco-2 whole cell. IHC: human bladder epithelial carcinoma tissue, human lung cancer tissue, human metaplasia of squamous cells of the renal pelvis tissue, human ovarian cancer tissue, human rectal moderately differentiated adenocarcinoma tissue. ICC/IF: SiHa cell. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acyl-CoA synthetase 4; Arachidonate--CoA ligase; fatty-acid-Coenzyme A ligase, long-chain 4; L MRX63; LACS 4; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 4; long-chain fatty-acid-Coenzyme A ligase 4; Long-chain-fatty-acid--CoA ligase 4
Gene Aliases: ACS4; ACSL4; FACL4; LACS4; MRX63; MRX68
UniProt ID: (Human) O60488
Entrez Gene ID: (Human) 2182
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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