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ACSL5 Antibody (PA5-114816) in ICC/IF
Immunocytochemistry analysis of ACSL5 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114816) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-114816
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ACSL5(Accession Q9ULC5), corresponding to amino acid residues P102-C152.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899452
Product Specific Information
Antibody detects endogenous levels of total ACSL5.
Target Information
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Arachidonate--CoA ligase; FACL5 for fatty acid coenzyme A ligase 5; fatty acid coenzyme A ligase 5; fatty acid Coenzyme A ligase, long chain 5; fatty-acid-Coenzyme A ligase, long-chain 5; HGNC:16526; LACS 5; Long-chain acyl-CoA synthetase 5; long-chain fatty acid coenzyme A ligase 5; Long-chain-fatty-acid--CoA ligase 5
Gene Aliases: 1700030F05Rik; ACS2; ACS5; ACSL5; FACL5; UNQ633/PRO1250
UniProt ID: (Human) Q9ULC5, (Rat) O88813, (Mouse) Q8JZR0
Entrez Gene ID: (Human) 51703, (Rat) 94340, (Mouse) 433256
long-chain fatty acid metabolic process
long-chain fatty-acyl-CoA biosynthetic process
fatty acid metabolic process
response to nutrient
metabolic process
phospholipid biosynthetic process
response to sucrose
response to glucose
positive regulation of plasma membrane long-chain fatty acid transport
positive regulation of triglyceride biosynthetic process
fatty acid transport
positive regulation of fatty acid beta-oxidation
cellular response to insulin stimulus
response to cholesterol
regulation of extrinsic apoptotic signaling pathway
lipid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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