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Invitrogen
ACTA1 Monoclonal Antibody (3H5)
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ACTA1 Antibody (MA5-49215) in IHC (P)
Immunohistochemistry analysis of ACTA1 in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with ACTA1 Monoclonal antibody (Product # MA5-49215) using a dilution of 5 μg/mL overnight at 4°C. Biotinylated goat anti-mouse IgG was ... View More
Product Details
MA5-49215
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3H5
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (277-308aa HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074873
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Immunogen sequence is identical to the related mouse and rat sequences.
Positive Control - WB: human HepG2 whole cell, human SK-OV-3 whole cell, human PANC-1 whole cell, human Hela whole cell, human A549 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell, mouse HEPA1-6 whole cell. IHC: human bladder cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin alpha 1; Actin, alpha skeletal muscle; Alpha-actin-1; nemaline myopathy type 3
Gene Aliases: AA959943; ACTA; Acta-2; ACTA1; Acts; Actsk-1; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; SHPM
UniProt ID: (Human) P68133, (Mouse) P68134, (Rat) P68136
Entrez Gene ID: (Human) 58, (Mouse) 11459, (Rat) 29437
muscle contraction
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
positive regulation of gene expression
cell growth
muscle filament sliding
skeletal muscle thin filament assembly
skeletal muscle fiber adaptation
response to steroid hormone
skeletal muscle fiber development
mesenchyme migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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