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ACTH Monoclonal Antibody (3C12C7)

ACTH Antibody in Immunocytochemistry (ICC/IF)

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ACTH Antibody (66358-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POMC antibody (Product # 66358-1-Ig) (Clone: 3C12C7) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

ACTH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

66358-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 7 publications 7 publications

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3C12C7

Immunogen

ACTH Fusion Protein Ag17311 (27-176 aa encoded by BC065832)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: WCLESSQCQ DLTTESNLLE CIRACKPDLS AETPMFPGNG DEQPLTENPR KYVMGHFRWD RFGRRNSSSS GSSGAGQKRE DVSAGEDCGP LPEGGPEPRS DGAKPGPREG KRSYSMEHFR WGKPVGKKRR PVKVYPNGAE DESAEAFPLE F (27-176 aa encoded by BC065832)

Target Information

ATCH (adrenocorticotropic hormone) is a hormone which plays a major role in stimulating the adrenal cortex. It is formed through cleavage of the polypeptide precursor proopiomelanocortin (POMC), which also results in several other cleavage products including MSH, ACTH, and beta endorphin. ATCH is secreted from the anterior pituitary in response to the corticotropin-releasing hormone from the hypothalamus. It stimulates the secretion of glucocorticoids like cortisol, but has little control over the stimulation of mineralocorticoids like aldosterone, which is another major hormone of the adrenal cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tumor-derived semaphorin 3D promoting cancer cachexia via regulating hypothalamic pro-opiomelanocortin neurons.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang Y,Xi Q,Zhong M,Jiang Y,Zhuang Q,Ding Z,Tan S,Wang J,Liu H,Zhang Z,Zhou B,Wu G

Thu Jun 01 00:00:00 UTC 2023

Sodium butyrate reduces overnutrition-induced microglial activation and hypothalamic inflammation.

International immunopharmacology

Wang X,Duan C,Li Y,Lu H,Guo K,Ge X,Chen T,Shang Y,Liu H,Zhang D

Sat Oct 01 00:00:00 UTC 2022

FumDSB can alleviate the inflammatory response induced by fumonisin B₁ in growing pigs.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment

Liu Q,Huang L,Cui Z,Qiao B,Li F,Wang C

Thu Sep 01 00:00:00 UTC 2022

FumDSB can alleviate the inflammatory response induced by fumonisin B₁ in growing pigs.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment

Liu Q,Huang L,Cui Z,Qiao B,Li F,Wang C

Thu Sep 01 00:00:00 UTC 2022

Gold nanoclusters eliminate obesity induced by antipsychotics.

Scientific reports

He M,Yao J,Zhang Z,Zhang Y,Chen R,Gu Z,Huang X,Deng C,Zhou R,Fan J,Zhang B,Xie Y,Gao G,Sun T

Fri Apr 01 00:00:00 UTC 2022

Gold nanoclusters eliminate obesity induced by antipsychotics.

Scientific reports

He M,Yao J,Zhang Z,Zhang Y,Chen R,Gu Z,Huang X,Deng C,Zhou R,Fan J,Zhang B,Xie Y,Gao G,Sun T

Fri Apr 01 00:00:00 UTC 2022

Elongator regulates the melanocortin satiety pathway.

Biochemical and biophysical research communications

Walters J,Walters C,Cameron B,George L

Tue Jul 12 00:00:00 UTC 2022

Elongator regulates the melanocortin satiety pathway.

Biochemical and biophysical research communications

Walters J,Walters C,Cameron B,George L

Tue Jul 12 00:00:00 UTC 2022

Involvement of POMC neurons in LEAP2 regulation of food intake and body weight.

Frontiers in endocrinology

Chu G,Peng H,Yu N,Zhang Y,Lin X,Lu Y

Tue Nov 22 00:00:00 UTC 2022

Involvement of POMC neurons in LEAP2 regulation of food intake and body weight.

Frontiers in endocrinology

Chu G,Peng H,Yu N,Zhang Y,Lin X,Lu Y

Tue Nov 22 00:00:00 UTC 2022

FoxO1 Regulates Neuropeptide Y and Pro-opiomelanocortin in the Hypothalamus of Rat Offspring Small for Gestational Age.

Reproductive sciences (Thousand Oaks, Calif.)

Zhang L,Shi Q,Sun Y

Sat Jan 01 00:00:00 UTC 2022

919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Xing JW,Chen MM,Tian XY,Pan DQ,Peng XH,Gao PF

Tue Jun 01 00:00:00 UTC 2021

Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma.

eLife

Zhang X,Lian P,Su M,Ji Z,Deng J,Zheng G,Wang W,Ren X,Jiang T,Zhang P,Li H

Tue Dec 14 00:00:00 UTC 2021

Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma.

eLife

Zhang X,Lian P,Su M,Ji Z,Deng J,Zheng G,Wang W,Ren X,Jiang T,Zhang P,Li H

Tue Dec 14 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: adrenal corticotropic hormone; adrenocorticotropic hormone; adrenocorticotropin; alpha-melanocyte stimulating hormone; alpha-melanocyte-stimulating hormone; alpha-MSH; beta-endorphin; beta-LPH; beta-melanocyte-stimulating hormone; beta-MSH; corticotropin-like intermediary peptide; Corticotropin-lipotropin; gamma-LPH; gamma-MSH; lipotropin beta; lipotropin gamma; melanotropin alpha; melanotropin beta; melanotropin gamma; met-enkephalin; opiomelanocortin prepropeptide; OTTHUMP00000119991; OTTHUMP00000200964; POMC; Precursor of MSH; pro-ACTH-endorphin; Pro-opiomelanocortin; pro-opiomelanocortin-alpha; proopiomelanocortin (adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin); proopiomelanocortin preproprotein; proopiomelanocortin, beta (endorphin, beta); proopoimelanocortin, beta (endorphin, beta)

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Gene Aliases: ACTH; alpha-MSH; alphaMSH; BE; Beta-LPH; beta-MSH; CLIP; Gamma-LPH; gamma-MSH; LPH; MSH; NPP; POC; POMC; Pomc-1; Pomc1; Pomc2

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UniProt ID: (Human) P01189, (Pig) P01192, (Mouse) P01193

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Entrez Gene ID: (Human) 5443, (Pig) 396863, (Mouse) 18976, (Rat) 24664

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Function(s)

G-protein coupled receptor binding receptor binding hormone activity neuropeptide hormone activity type 3 melanocortin receptor binding type 4 melanocortin receptor binding type 1 melanocortin receptor binding molecular_function

Process(es)

generation of precursor metabolites and energy signal transduction neuropeptide signaling pathway cell-cell signaling regulation of blood pressure regulation of appetite negative regulation of tumor necrosis factor production cellular pigmentation glucose homeostasis positive regulation of transcription from RNA polymerase II promoter regulation of glycogen metabolic process regulation of corticosterone secretion feeding behavior peptide hormone processing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ACTH Monoclonal Antibody (3C12C7)

ACTH Antibody (66358-1-IG) in ICC/IF

Product Details

66358-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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