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ADO Antibody (PA5-78733) in ICC/IF
Immunocytochemistry analysis of ADO using anti-ADO antibody (Product # PA5-78733) . ADO was detected in a section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-ADO antibody (Product # PA5-78733) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize... View More
Product Details
PA5-78733
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human ADO recombinant protein (Position: E49-E261).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745849
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human PC-3 whole cell, human MCF-7 whole cell, rat brain tissue, mouse heart tissue, mouse brain tissue. IHC: rat brain tissue, mouse brain tissue. ICC/IF: A549 cell. Flow: A549 cell, PC-3 cell, U251 cell, Hela cell.
Target Information
Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-amino ethane thiol) dioxygenase (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-aminoethanethiol (cysteamine) dioxygenase; 2-aminoethanethiol dioxygenase; cysteamine (2-aminoethanethiol) dioxygenase (ADO); Cysteamine dioxygenase
Gene Aliases: ADO; C10orf22; Gm237
UniProt ID: (Human) Q96SZ5, (Mouse) Q6PDY2
Entrez Gene ID: (Human) 84890, (Mouse) 211488
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