Proteintech

AEG-1/MTDH Polyclonal Antibody

AEG-1/MTDH Antibody in Immunocytochemistry (ICC/IF)

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AEG-1/MTDH Antibody (13860-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using MTDH antibody 13860-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

AEG-1/MTDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AEG-1/MTDH Antibody in Western Blot (WB)

AEG-1/MTDH Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13860-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 36 publications 36 publications

Immunohistochemistry (IHC)

View 22 publications 22 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AEG-1/MTDH-Specific Fusion Protein Ag4840 (233-582 aa encoded by BC045642)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.

Immunogen sequence: EQLTTASFP VGSKKNKGDS HLNVQVSNFK SGKGDSTLQV SSGLNENLTV NGGGWNEKSV KLSSQISAGE EKWNSVSPAS AGKRKAEPSA WSQDTGDANT NGKDWGRSWS DRSIFSGIGS TAEPVSQSTT SDYQWDVSRN QPYIDDEWSG LNGLSSADPN SDWNAPAEEW GNWVDEERAS LLKSQEPIPD DQKVSDDDKE KGEGALPTGK SKKKKKKKKK QGEDNSTAQD TEELEKEIRE DLPVNTSKTR PKQEKAFSLK TISTSDPAEV LVKNSQPIKT LPPATSTEPS VILSKSDSDK SSSQVPPILQ ETDKSKSNTK QNSVPPSQTK SETSWESPKQ IKKKKKARRE T (233-582 aa encoded by BC045642)

Target Information

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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International immunopharmacology

Zhang Z,Zhu Z,Fu J,Liu X,Mi Z,Tao H,Fan H

Wed Feb 01 00:00:00 UTC 2023

Anoikis patterns exhibit distinct prognostic and immune landscapes in Osteosarcoma.

International immunopharmacology

Zhang Z,Zhu Z,Fu J,Liu X,Mi Z,Tao H,Fan H

Wed Feb 01 00:00:00 UTC 2023

Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2.

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Fu J,Peng J,Tu G

Fri Sep 23 00:00:00 UTC 2022

Microparticles from Hyperphosphatemia-Stimulated Endothelial Cells Promote Vascular Calcification Through Astrocyte-Elevated Gene-1.

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Xiang Y,Duan Y,Peng Z,Huang H,Ding W,Chen E,Liu Z,Dou C,Li J,Ou J,Wan Q,Yang B,He Z

Fri Jul 01 00:00:00 UTC 2022

Downregulation of Astrocyte Elevated Gene-1 Expression Combined with All-Trans Retinoic Acid Inhibits Development of Vasculogenic Mimicry and Angiogenesis in Glioma.

Current medical science

Liang C,Yang L,Guo SW,Li RC

Fri Apr 01 00:00:00 UTC 2022

Transcriptome analysis in an AEG‑1‑deficient neuronal HT22 cell line.

Experimental and therapeutic medicine

Liu K,Wan P,Huang Y,Wang B,Wang X,Zhang R,Guo L

Tue Nov 01 00:00:00 UTC 2022

Miat and interacting protein Metadherin maintain a stem-like niche to promote medulloblastoma tumorigenesis and treatment resistance.

Proceedings of the National Academy of Sciences of the United States of America

Peng KL,Vasudevan HN,Lockney DT,Baum R,Hendrickson RC,Raleigh DR,Schmitt AM

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MTDH Promotes Intestinal Inflammation by Positively Regulating TLR Signalling.

Journal of Crohn's & colitis

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Sat Dec 18 00:00:00 UTC 2021

MTDH Promotes Intestinal Inflammation by Positively Regulating TLR Signalling.

Journal of Crohn's & colitis

Wang L,Zhang N,Han D,Su P,Chen B,Zhao W,Liu Y,Zhang H,Hu G,Yang Q

Sat Dec 18 00:00:00 UTC 2021

MTDH Promotes Intestinal Inflammation by Positively Regulating TLR Signalling.

Journal of Crohn's & colitis

Wang L,Zhang N,Han D,Su P,Chen B,Zhao W,Liu Y,Zhang H,Hu G,Yang Q

Sat Dec 18 00:00:00 UTC 2021

MTDH Promotes Intestinal Inflammation by Positively Regulating TLR Signalling.

Journal of Crohn's & colitis

Wang L,Zhang N,Han D,Su P,Chen B,Zhao W,Liu Y,Zhang H,Hu G,Yang Q

Sat Dec 18 00:00:00 UTC 2021

Downregulation of astrocyte elevated gene-1 expression inhibits the development of vasculogenic mimicry in gliomas.

Experimental and therapeutic medicine

Liang C,Shangguan J,Yang L,Guo S

Fri Jan 01 00:00:00 UTC 2021

Downregulation of Long Noncoding RNA TUG1 Attenuates MTDH-Mediated Inflammatory Damage via Targeting miR-29b-1-5p After Spinal Cord Ischemia Reperfusion.

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Thu Jul 01 00:00:00 UTC 2021

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Mon Aug 30 00:00:00 UTC 2021

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Scientific reports

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Mon Aug 30 00:00:00 UTC 2021

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Scientific reports

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Mon Aug 30 00:00:00 UTC 2021

Overcoming cisplatin resistance by targeting the MTDH-PTEN interaction in ovarian cancer with sera derived from rats exposed to Guizhi Fuling wan extract.

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Mon Feb 17 00:00:00 UTC 2020

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Biochemistry and cell biology = Biochimie et biologie cellulaire

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CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH-NF-κB signalling pathway.

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Thu Mar 01 00:00:00 UTC 2018

Metadherin contributes to epithelial-mesenchymal transition and paclitaxel resistance induced by acidic extracellular pH in nasopharyngeal carcinoma.

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Wed Jun 26 00:00:00 UTC 2019

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Chen X,Li XY,Long M,Wang X,Gao ZW,Cui Y,Ren J,Zhang Z,Liu C,Dong K,Zhang H

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Wed Nov 01 00:00:00 UTC 2017

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Wed Nov 01 00:00:00 UTC 2017

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Mon Mar 21 00:00:00 UTC 2022

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Thu Jun 15 00:00:00 UTC 2017

Knockdown of astrocyte elevated gene-1 inhibited cell growth and induced apoptosis and suppressed invasion in ovarian cancer cells.

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Mon May 01 00:00:00 UTC 2017

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Mon May 01 00:00:00 UTC 2017

MiR-98 inhibits malignant progression via targeting MTDH in squamous cell carcinoma of the head and neck.

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miR-217 inhibits laryngeal cancer metastasis by repressing AEG-1 and PD-L1 expression.

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Mon Feb 19 00:00:00 UTC 2018

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