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FIGURE: 1 / 1
AF4 Antibody (34042-100UG) in WB
Immunoblotting: use at 1-5 µg/mL. A band of ~131 kDa is detected. IP western blot analysis was performed on human leukemia cell lines that harbor MLL chromosomal translocations and express MLL chimeric oncoproteins (indicated at tops). IP was performed with the antibodies indicated on the top of the gels, and the precipitates were immunoblotted with the antibodies indicated on the right. Antibody #34042 is shown as AF4. Anti-GST antibodies served as negative controls.
Product Details
34042-100UG
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
C2.1.1
Immunogen
Maltose-binding fusion protein containing AF4 amino acids 782-979 (Accession no. NP_005926).
Conjugate
Form
Liquid
Concentration
0.5-1.0 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Proto-oncogene AF-4 (or FEL) is a product of a chromosomal aberration of the human gene AFF1, which is associated with acute leukemias. The fusion of AF-4 on chromosome band 4q21 with the mixed lineage leukemia (MLL or HRX) gene on 11q23 results in a MLL-AF-4 chimeric transcription factor in which AF-4 contributes transcriptional effector properties and requires cell-specific accessory factors. MLL is involved in several chromosomal translocations associated with acute myeloid and lymphoid leukemia. The MLL-AF-4 fusion protein is expressed in all normal hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AF4/FMR2 family member 1; AF4/FMR2 family, member 1; ALL1-fused gene from chromosome 4 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2; pre-B-cell monocytic leukemia partner 1; Protein AF-4; Protein FEL; Proto-oncogene AF4
Gene Aliases: AF4; AFF1; FEL; MLLT2; PBM1
UniProt ID: (Human) P51825
Entrez Gene ID: (Human) 4299
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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