Proteintech

AFG3L2 Polyclonal Antibody

AFG3L2 Antibody in Immunocytochemistry (ICC/IF)

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AFG3L2 Antibody (14631-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

AFG3L2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AFG3L2 Antibody in Immunoprecipitation (IP)

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Product Details

14631-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 22 publications 22 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AFG3L2 Fusion Protein Ag6209 (449-797 aa encoded by BC065016)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ILAGTNRPD ILDPALLRPG RFDRQIFIGP PDIKGRASIF KVHLRPLKLD STLEKDKLAR KLASLTPGFS GADVANVCNE AALIAARHLS DSINQKHFEQ AIERVIGGLE KKTQVLQPEE KKTVAYHEAG HAVAGWYLEH ADPLLKVSII PRGKGLGYAQ YLPKEQYLYT KEQLLDRMCM TLGGRVSEEI FFGRITTGAQ DDLRKVTQSA YAQIVQFGMN EKVGQISFDL PRQGDMVLEK PYSEATARLI DDEVRILIND AYKRTVALLT EKKADVEKVA LLLLEKEVLD KNDMVELLGP RPFAEKSTYE EFVEGTGSLD EDTSLPEGLK DWNKEREKEK EEPPGEKVAN (449-797 aa encoded by BC065016)

Target Information

AFG3L2 is a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. AFG3L2 gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders. This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Tue Jun 20 00:00:00 UTC 2023

Ferroptosis inducers enhanced cuproptosis induced by copper ionophores in primary liver cancer.

Journal of experimental & clinical cancer research : CR

Wang W,Lu K,Jiang X,Wei Q,Zhu L,Wang X,Jin H,Feng L

Tue Jun 06 00:00:00 UTC 2023

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 UTC 2022

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 UTC 2022

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 UTC 2022

Prohibitin 1 regulates mtDNA release and downstream inflammatory responses.

The EMBO journal

Liu H,Fan H,He P,Zhuang H,Liu X,Chen M,Zhong W,Zhang Y,Zhen C,Li Y,Jiang H,Meng T,Xu Y,Zhao G,Feng D

Thu Dec 15 00:00:00 UTC 2022

The mitochondrial UPR regulator ATF5 promotes intestinal barrier function via control of the satiety response.

Cell reports

Chamseddine D,Mahmud SA,Westfall AK,Castoe TA,Berg RE,Pellegrino MW

Tue Dec 13 00:00:00 UTC 2022

Leber's hereditary optic neuropathy-associated ND6 14484T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy.

Human molecular genetics

Liang M,Ji Y,Zhang L,Wang X,Hu C,Zhang J,Zhu Y,Mo JQ,Guan MX

Thu Sep 29 00:00:00 UTC 2022

Translation of MT-ATP6 pathogenic variants reveals distinct regulatory consequences from the co-translational quality control of mitochondrial protein synthesis.

Human molecular genetics

Ng KY,Richter U,Jackson CB,Seneca S,Battersby BJ

Fri Apr 22 00:00:00 UTC 2022

Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex.

Molecular cell

Tsai CW,Rodriguez MX,Van Keuren AM,Phillips CB,Shushunov HM,Lee JE,Garcia AM,Ambardekar AV,Cleveland JC,Reisz JA,Proenza C,Chatfield KC,Tsai MF

Thu Oct 06 00:00:00 UTC 2022

Complementary anti-cancer pathways triggered by inhibition of sideroflexin 4 in ovarian cancer.

Scientific reports

Tesfay L,Paul BT,Hegde P,Brewer M,Habbani S,Jellison E,Moore T,Wu H,Torti SV,Torti FM

Sat Nov 19 00:00:00 UTC 2022

A novel mutation located in the intermembrane space domain of AFG3L2 causes dominant optic atrophy through decreasing the stability of the encoded protein.

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Yang L,Jin X,Li Y,Guo Q,Yang M,You Y,Yao S,Zhang X,Wang Z,Lei B

Mon Aug 15 00:00:00 UTC 2022

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Cellular and molecular gastroenterology and hepatology

Sun D,Niu Z,Zheng HX,Wu F,Jiang L,Han TQ,Wei Y,Wang J,Jin L

Mon Mar 07 00:00:00 UTC 2022

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Annals of clinical and translational neurology

Dosi C,Galatolo D,Rubegni A,Doccini S,Pasquariello R,Nesti C,Sicca F,Barghigiani M,Battini R,Tessa A,Santorelli FM

Wed Apr 01 00:00:00 UTC 2020

Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNA^His mutation.

The Journal of biological chemistry

Gong S,Wang X,Meng F,Cui L,Yi Q,Zhao Q,Cang X,Cai Z,Mo JQ,Liang Y,Guan MX

Fri Jan 24 00:00:00 UTC 2020

Sphingosine kinase 1 overexpression induces MFN2 fragmentation and alters mitochondrial matrix Ca²⁺ handling in HeLa cells.

Biochimica et biophysica acta. Molecular cell research

Pulli I,Löf C,Blom T,Asghar MY,Lassila T,Bäck N,Lin KL,Nyström JH,Kemppainen K,Toivola DM,Dufour E,Sanz A,Cooper HM,Parys JB,Törnquist K

Sun Sep 01 00:00:00 UTC 2019

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Nucleic acids research

Chen D,Zhang Z,Chen C,Yao S,Yang Q,Li F,He X,Ai C,Wang M,Guan MX

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Fidelity of translation initiation is required for coordinated respiratory complex assembly.

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Rudler DL,Hughes LA,Perks KL,Richman TR,Kuznetsova I,Ermer JA,Abudulai LN,Shearwood AJ,Viola HM,Hool LC,Siira SJ,Rackham O,Filipovska A

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Mitochondrial stress response triggered by defects in protein synthesis quality control.

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Richter U,Ng KY,Suomi F,Marttinen P,Turunen T,Jackson C,Suomalainen A,Vihinen H,Jokitalo E,Nyman TA,Isokallio MA,Stewart JB,Mancini C,Brusco A,Seneca S,Lombès A,Taylor RW,Battersby BJ

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Sideroflexin 4 affects Fe-S cluster biogenesis, iron metabolism, mitochondrial respiration and heme biosynthetic enzymes.

Scientific reports

Paul BT,Tesfay L,Winkler CR,Torti FM,Torti SV

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Cell reports

Jian F,Chen D,Chen L,Yan C,Lu B,Zhu Y,Chen S,Shi A,Chan DC,Song Z

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Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Cell reports

Jian F,Chen D,Chen L,Yan C,Lu B,Zhu Y,Chen S,Shi A,Chan DC,Song Z

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Proteolytic control of the mitochondrial calcium uniporter complex.

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Tsai CW,Wu Y,Pao PC,Phillips CB,Williams C,Miller C,Ranaghan M,Tsai MF

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Proteolytic control of the mitochondrial calcium uniporter complex.

Proceedings of the National Academy of Sciences of the United States of America

Tsai CW,Wu Y,Pao PC,Phillips CB,Williams C,Miller C,Ranaghan M,Tsai MF

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Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Science advances

Perks KL,Ferreira N,Richman TR,Ermer JA,Kuznetsova I,Shearwood AJ,Lee RG,Viola HM,Johnstone VPA,Matthews V,Hool LC,Rackham O,Filipovska A

Tue Aug 01 00:00:00 UTC 2017

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Science advances

Perks KL,Ferreira N,Richman TR,Ermer JA,Kuznetsova I,Shearwood AJ,Lee RG,Viola HM,Johnstone VPA,Matthews V,Hool LC,Rackham O,Filipovska A

Tue Aug 01 00:00:00 UTC 2017

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

The Journal of cell biology

Richter U,Lahtinen T,Marttinen P,Suomi F,Battersby BJ

Mon Oct 26 00:00:00 UTC 2015

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

The Journal of cell biology

Richter U,Lahtinen T,Marttinen P,Suomi F,Battersby BJ

Mon Oct 26 00:00:00 UTC 2015

Novel MTND1 mutations cause isolated exercise intolerance, complex I deficiency and increased assembly factor expression.

Clinical science (London, England : 1979)

Gorman GS,Blakely EL,Hornig-Do HT,Tuppen HA,Greaves LC,He L,Baker A,Falkous G,Newman J,Trenell MI,Lecky B,Petty RK,Turnbull DM,McFarland R,Taylor RW

Mon Jun 01 00:00:00 UTC 2015

Novel MTND1 mutations cause isolated exercise intolerance, complex I deficiency and increased assembly factor expression.

Clinical science (London, England : 1979)

Gorman GS,Blakely EL,Hornig-Do HT,Tuppen HA,Greaves LC,He L,Baker A,Falkous G,Newman J,Trenell MI,Lecky B,Petty RK,Turnbull DM,McFarland R,Taylor RW

Mon Jun 01 00:00:00 UTC 2015

Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.

Brain : a journal of neurology

Pfeffer G,Gorman GS,Griffin H,Kurzawa-Akanbi M,Blakely EL,Wilson I,Sitarz K,Moore D,Murphy JL,Alston CL,Pyle A,Coxhead J,Payne B,Gorrie GH,Longman C,Hadjivassiliou M,McConville J,Dick D,Imam I,Hilton D,Norwood F,Baker MR,Jaiser SR,Yu-Wai-Man P,Farrell M,McCarthy A,Lynch T,McFarland R,Schaefer AM,Turnbull DM,Horvath R,Taylor RW,Chinnery PF

Thu May 01 00:00:00 UTC 2014

Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.

Brain : a journal of neurology

Thu May 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: AFG3 ATPase family gene 3-like 2; AFG3 ATPase family member 3-like 2; AFG3 like AAA ATPase 2; AFG3(ATPase family gene 3)-like 2; AFG3-like AAA ATPase 2; AFG3-like protein 2; ATPase family gene 3, yeast; FLJ25993; Mitochondrial inner membrane m-AAA protease component AFG3L2; Paraplegin-like protein

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Gene Aliases: 2310036I02Rik; AFG3L2; AW260507; Emv66; par; SCA28; SPAX5

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UniProt ID: (Human) Q9Y4W6, (Mouse) Q8JZQ2

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Entrez Gene ID: (Human) 10939, (Mouse) 69597, (Rat) 307350

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Function(s)

ATP-dependent peptidase activity metalloendopeptidase activity protein binding ATP binding metallopeptidase activity zinc ion binding unfolded protein binding nucleotide binding peptidase activity hydrolase activity metal ion binding

Process(es)

proteolysis mitochondrion organization axonogenesis neuromuscular junction development mitochondrial fusion obsolete death nerve development mitochondrial protein processing regulation of multicellular organism growth cristae formation myelination muscle fiber development righting reflex protein complex assembly protein import into mitochondrial intermembrane space

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AFG3L2 Polyclonal Antibody

AFG3L2 Antibody (14631-1-AP) in ICC/IF

Product Details

14631-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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