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Invitrogen
AGFG1 Polyclonal Antibody
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FIGURE: 1 / 5
AGFG1 Antibody (PA5-30234) in ICC/IF
Immunofluorescence analysis of AGFG1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with AGFG1 Polyclonal Antibody (Product # PA5-30234) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody (Product # A27034) at a dilution of 1:20... View More
Product Details
PA5-30234
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human RIP/Rab. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547708
Product Specific Information
Recommended positive controls: IMR32.
Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (88%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HRB is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. HRB binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. This encoded protein binds the Rev activation domain when Rev is assembled onto its RNA target and can significantly enhance Rev activity when overexpressed. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Arf-GAP domain and FG repeat-containing protein 1; arf-GAP domain and FG repeats-containing protein 1; HIV-1 Rev binding protein; HIV-1 Rev-binding protein; HIV-1 Rev-binding protein homolog; hRIP, Rev interacting protein; MGC116938; MGC116940; Nucleoporin-like protein RIP; Rab, Rev/Rex activation domain-binding protein; Rev-interacting protein; Rev/Rex activation domain-binding protein
Gene Aliases: AGFG1; AU045498; C130049H11Rik; C85612; D730048C23Rik; HRB; RAB; RIP
UniProt ID: (Human) P52594, (Mouse) Q8K2K6
Entrez Gene ID: (Human) 3267, (Mouse) 15463
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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