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Invitrogen
AGO1 Recombinant Rabbit Monoclonal Antibody (6H1L4)
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FIGURE: 1 / 3
AGO1 Antibody (702720) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), NTERA-2 cl.D1 (Lane 2), HEL 92.1.7 (Lane 3), K-562 (Lane 4), MOLT4 (Lane 5), HL-60 (Lane 6), A-431 (Lane 7) and THP-1 (Lane 8). The blots were probed with Anti-AGO1 Recombinant Rabbit Monoclonal Antibody (Product # 702720, 1 µg/mL). A 97 kDa band corresponding to AGO1 was observed across the cell lines tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Produc... View More
Product Details
702720
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H1L4
Immunogen
Peptides corresponding to human AGO1 (1) aa17-aa35 2) aa382-aa401)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2692255
Product Specific Information
This antibody is predicted to react with Monkey, Pig, and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
AGO1 is a member of the Argonaute family of proteins which play a role in RNA interference. The protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. AGO1 binds to miRNAs or siRNAs and represses the translation of mRNAs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: argonaute 1; Argonaute RISC catalytic component 1; Argonaute1; eIF-2C 1; eIF2C 1; Eukaryotic translation initiation factor 2C 1; eukaryotic translation initiation factor 2C, 1; Golgi Endoplasmic Reticulum protein 95 kDa; Protein argonaute-1; Putative RNA-binding protein Q99
Gene Aliases: AGO1; EIF2C; EIF2C1; GERP95; hAgo1; Q99
UniProt ID: (Human) Q9UL18
Entrez Gene ID: (Human) 26523
nuclear-transcribed mRNA catabolic process
transcription, DNA-templated
Wnt signaling pathway, calcium modulating pathway
RNA secondary structure unwinding
miRNA metabolic process
gene silencing by RNA
pre-miRNA processing
posttranscriptional gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA cleavage involved in gene silencing by miRNA
miRNA loading onto RISC involved in gene silencing by miRNA
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol-mediated signaling
mRNA cleavage involved in gene silencing by siRNA
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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