Invitrogen

AGO2 Monoclonal Antibody (AGO2 11A9), eBioscience™

AGO2 Antibody in Immunocytochemistry (ICC/IF)

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AGO2 Antibody (14-6519-82) in ICC/IF

Immunofluorescent analysis of Ago2 (green) in U-2 OS cells (45% confluency). The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 1% Triton X-100 for 30 minutes at 37 C and blocked with 10% Normal Goat Serum in PBS with 0.1% Triton X-100 for 1 hour at room temperature. Cells were stained with the Ago2 Monoclonal Antibody (Product # 14-6519-82) at a concentration of 1.25 µg/mL or Rat IgG2a Isotype Control at 4 C overnight in blocking buffer followed by a Goat anti-Rat IgG (H+L) Superclonal Sec... View More

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AGO2 Antibody — Western Blot analysis was performed using whole cell extracts of wild type (WT) 293T cells (Lane 1) and Ago2 knockout 293T cells generated by Crispr/Cas9 gene targeting (Lane 2) on a 4-12% Nupage Bis-Tris Gel (Product # NP0336BOX) for gel electrophoresis in MOPS SDS Running Buffer (Product # NP0001-02). Protein transfer to a nitrocellulose membrane was achieved using the iBlot 2 system. The membrane was probed with Ago2 Monoclonal Antibody (clone AGO2 11A9, 4 µg/mL) and detected by chemiluminescence using Goat-anti Rat IgG Secondary Antibody, HRP (Product # 31470) at 1:10,000 dilution. This Ago2 Monoclonal Antibody detects a 93 kDa band in WT 293T cells but not in Ago2 knockout lysate using Super Signal West Pico chemiluminescence substrate. Protein size was determined using Page Ruler Pre-stained Protein Standard (Product # 26616). {KO}

Product Details

14-6519-82

Applications

Tested Dilution

Publications

Western Blot (WB)

4.0 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1.25 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

AGO2 11A9

Immunogen

Peptide of human N-terminus

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2784637

Product Specific Information

Description: This AGO2 11A9 monoclonal antibody recognizes human Argonaut 2 (Ago2), which has been described as a transcriptional regulator by means of RNA interference and endonucleolytic cleavage. The epitope recognized by AGO2 11A9 detects reduced Ago2 by Western Blot and stains Ago2 in the cytoplasm and nucleus in Immunocytochemistry. Ago2 monoclonal antibody (AGO2 11A9) has been validated for the following applications: Western Blot and Immunocytochemistry; AGO2 11A9 does not cross react with murine Ago2.

Applications Reported: This AGO2 11A9 antibody has been reported for use in western blot and immunocytochemistry/immunofluorescence.

Applications Tested: This AGO2 11A9 antibody has been tested by western blot using lysates from different human cancer cell lines and by immunocytochemistry/immunofluorescence using fixed human U-2 OS cells. This may be used at less than or equal to 4 µg/mL for western blot and at less than or equal to 1.25 µg/mL for immunocytochemistry/immunofluorescence.

Target Information

Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or ″Slicer″ activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Silencing of Ago-2 Interacting Protein SERBP1 Relieves KCC2 Repression by miR-92 in Neurons.

Cells

Barbato C,Frisone P,Braccini L,D'Aguanno S,Pieroni L,Ciotti MT,Catalanotto C,Cogoni C,Ruberti F

Published figure using AGO2 monoclonal antibody (Product # 14-6519-82) in Western Blot

Sun Mar 20 00:00:00 UTC 2022

No evidence for Ago2 translocation from the host erythrocyte into the Plasmodium parasite.

Wellcome open research

Hentzschel F,Obrova K,Marti M

14-6519-82 was used in Immunocytochemistry to show that previously observed Ago2 localisation in the parasite by fluorescence microscopy is the result of antibody cross reactivity.

Sun Mar 31 00:00:00 UTC 2024

No evidence for Ago2 translocation from the host erythrocyte into the Plasmodium parasite.

Wellcome open research

Hentzschel F,Obrova K,Marti M

14-6519-82 was used in Immunocytochemistry to show that previously observed Ago2 localisation in the parasite by fluorescence microscopy is the result of antibody cross reactivity.

Sun Mar 31 00:00:00 UTC 2024

Bioinformatics

Protein Aliases: AGO1; argonaute 2; Argonaute RISC catalytic component 2; Argonaute1; Argonaute2; CTA-204B4.6; eIF-2C 1; eIF-2C 2; eIF2C; eIF2C 1; eIF2C 2; EIF2C1; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C, 2; hAgo1; hAgo2; PAZ Piwi domain protein; PPD; Protein argonaute-1; Protein argonaute-2; Protein slicer

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Gene Aliases: AGO2; EIF2C2; Q10

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UniProt ID: (Human) Q9UKV8

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Entrez Gene ID: (Human) 27161

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Function(s)

RNA 7-methylguanosine cap binding RNA polymerase II core binding core promoter binding double-stranded RNA binding single-stranded RNA binding mRNA binding translation initiation factor activity endoribonuclease activity protein binding protein C-terminus binding siRNA binding miRNA binding poly(A) RNA binding metal ion binding endoribonuclease activity, cleaving siRNA-paired mRNA endoribonuclease activity, cleaving miRNA-paired mRNA

Process(es)

transcription, DNA-templated translation translational initiation Wnt signaling pathway, calcium modulating pathway post-embryonic development RNA secondary structure unwinding miRNA metabolic process gene silencing by RNA pre-miRNA processing siRNA loading onto RISC involved in RNA interference posttranscriptional gene silencing by RNA production of miRNAs involved in gene silencing by miRNA miRNA mediated inhibition of translation mRNA cleavage involved in gene silencing by miRNA miRNA loading onto RISC involved in gene silencing by miRNA positive regulation of transcription from RNA polymerase II promoter negative regulation of translational initiation phosphatidylinositol-mediated signaling positive regulation of nuclear-transcribed mRNA poly(A) tail shortening RNA phosphodiester bond hydrolysis, endonucleolytic mRNA cleavage involved in gene silencing by siRNA positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AGO2 Monoclonal Antibody (AGO2 11A9), eBioscience™

AGO2 Antibody (14-6519-82) in ICC/IF

Product Details

14-6519-82

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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