Invitrogen

AGO2 Monoclonal Antibody (R.386.2)

FIGURE: 1 / 1

AGO2 Antibody (MA5-14861) in WB

Western blot was performed using anti-AGO2 Monoclonal Antibody (R.386.2) (Product # MA5-14861) and a band at 97 kDa corresponding to AGO2 was observed across the cell lines tested and was increased upon LPS treatment in THP-1. An uncharacterized band (*) was also observed at ~20 kDa. Modified whole cell extracts (1% SDS) (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), T-47D (Lane 3), Hep G2 (Lane 4), THP-1 (Lane 5), THP-1 treated with LPS (1 µg/mL for 24hrs) (Lane 6) and RAW 264.7 (Lane 7) were electrophoresed using Novex® NuPAGE... View More

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Product Details

MA5-14861

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:50

View 3 publications 3 publications

RNA Immunoprecipitation (RIP)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

R.386.2

Immunogen

Synthetic peptide corresponding to mouse Argonaute 2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

260 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10980123

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or ″Slicer″ activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Combined high-throughput library screening and next generation RNA sequencing uncover microRNAs controlling human cardiac fibroblast biology.

Journal of molecular and cellular cardiology

Schimmel K,Stojanović SD,Huang CK,Jung M,Meyer MH,Xiao K,Grote-Levi L,Bär C,Pfanne A,Mitzka S,Just A,Geffers R,Bock K,Kenneweg F,Kleemiß F,Falk CS,Fiedler J,Thum T

MA5-14861 was used in Immunoprecipitation to provide evidence that repression of autophagy and detoxification of reactive oxygen species by miR-20a-5p and miR-132-3p explain some of their pro-fibrotic nature on a mechanistic level.

Fri Jan 01 00:00:00 UTC 2021

Long non‑coding RNA AC245100.4 promotes prostate cancer tumorigenesis via the microRNA‑145‑5p/RBBP5 axis.

Oncology reports

Xie H,Zhao J,Wan J,Zhao J,Wang Q,Yang X,Yang W,Lin P,Yu X

MA5-14861 was used in RNA Immunoprecipitation to investigate the overall biological function, mechanism of action and clinical importance of lncRNA AC245100.4 in prostate cancer (PCa).

Mon Feb 01 00:00:00 UTC 2021

LncRNA PCAT6 regulates the progression of pituitary adenomas by regulating the miR-139-3p/BRD4 axis.

Cancer cell international

Zhao P,Cheng J,Li B,Nie D,Wang H,Li C,Gui S,Zhang Y

MA5-14861 was used in RNA Immunoprecipitation to conclude that PCAT6 regulated the progression of PA via modulating the miR-139-3p/BRD4 axis, which might provide a novel biomarker for the prevention, diagnosis, and treatment of PA.

Wed Jan 06 00:00:00 UTC 2021

Long non-coding RNA SNHG8 enhances triple-negative breast cancer cell proliferation and migration by regulating the miR-335-5p/PYGO2 axis.

Biology direct

Qian J,Lei X,Sun Y,Zheng L,Li J,Zhang S,Zhang L,Li W,Shi J,Jia W,Tang T

MA5-14861 was used in RNA Immunoprecipitation to investigate the role of long non-coding small nucleolar host gene 8 in the proliferation and migration of triple-negative breast cancer cells.

Fri Aug 06 00:00:00 UTC 2021

Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Promote Neuroprotection in Rodent Models of Glaucoma.

Investigative ophthalmology & visual science

Mead B,Amaral J,Tomarev S

MA5-14861 was used in Western Blot to investigate the benefit of bone marrow mesenchymal stem cell (BMSC)-derived small extracellular vesicles (sEV) as an intravitreal (ivit) therapy in two rat models of glaucoma and to determine and identify candidate miRNA involved in the mechanism.

Thu Feb 01 00:00:00 UTC 2018

miR-21 promotes fibrosis in an acute cardiac allograft transplantation model.

Cardiovascular research

Gupta SK,Itagaki R,Zheng X,Batkai S,Thum S,Ahmad F,Van Aelst LN,Sharma A,Piccoli MT,Weinberger F,Fiedler J,Heuser M,Heymans S,Falk CS,Förster R,Schrepfer S,Thum T

MA5-14861 was used in immunoprecipitation to elucidate miR-21 that induces fibrosis in an acute cardiac allograft transplantation model

Sun May 15 00:00:00 UTC 2016

Circular non-coding RNA ANRIL modulates ribosomal RNA maturation and atherosclerosis in humans.

Nature communications

Holdt LM,Stahringer A,Sass K,Pichler G,Kulak NA,Wilfert W,Kohlmaier A,Herbst A,Northoff BH,Nicolaou A,Gäbel G,Beutner F,Scholz M,Thiery J,Musunuru K,Krohn K,Mann M,Teupser D

MA5-14861 was used in RNA immunoprecipitation identify an atheroprotective role for circular RNAs

Fri Aug 19 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: AGO1; argonaute 2; Argonaute RISC catalytic component 2; Argonaute1; Argonaute2; argonaute2 {ECO:0000255|HAMAP-Rule:MF_03031}; CTA-204B4.6; eIF-2C 1; eIF-2C 2; eIF-2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; eIF2C; eIF2C 1; eIF2C 2; eIF2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; EIF2C1; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}; eukaryotic translation initiation factor 2C, 2; GERp95; golgi ER protein 95 kDa; hAgo1; hAgo2; mAgo2; PAZ Piwi domain protein; Piwi/Argonaute family protein meIF2C2; PPD; Protein argonaute-1; Protein argonaute-2; Protein slicer

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Gene Aliases: 1110029L17Rik; 2310051F07Rik; AGO2; AI225898; AL022874; AW546247; EIF2C2; ENSMUSG00000072493; Gerp95; Gm10365; Kiaa4215; mKIAA4215; Q10

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UniProt ID: (Human) Q9UKV8, (Mouse) Q8CJG0

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Entrez Gene ID: (Human) 27161, (Rat) 59117, (Mouse) 239528

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Function(s)

RNA 7-methylguanosine cap binding RNA polymerase II core binding core promoter binding double-stranded RNA binding single-stranded RNA binding mRNA binding translation initiation factor activity endoribonuclease activity protein binding protein C-terminus binding siRNA binding miRNA binding poly(A) RNA binding metal ion binding endoribonuclease activity, cleaving siRNA-paired mRNA endoribonuclease activity, cleaving miRNA-paired mRNA molecular_function RNA binding pre-miRNA binding nucleic acid binding nuclease activity endonuclease activity hydrolase activity

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated mRNA cleavage biological_process post-embryonic development miRNA metabolic process positive regulation of gene expression cell differentiation gene silencing by RNA pre-miRNA processing miRNA mediated inhibition of translation mRNA cleavage involved in gene silencing by miRNA miRNA loading onto RISC involved in gene silencing by miRNA negative regulation of translational initiation positive regulation of nuclear-transcribed mRNA poly(A) tail shortening RNA phosphodiester bond hydrolysis, endonucleolytic positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay regulation of translation multicellular organism development RNA secondary structure unwinding siRNA loading onto RISC involved in RNA interference production of miRNAs involved in gene silencing by miRNA positive regulation of transcription from RNA polymerase II promoter mRNA cleavage involved in gene silencing by siRNA translation translational initiation Wnt signaling pathway, calcium modulating pathway posttranscriptional gene silencing by RNA phosphatidylinositol-mediated signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AGO2 Monoclonal Antibody (R.386.2)

AGO2 Antibody (MA5-14861) in WB

Product Details

MA5-14861

Western Blot (WB)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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