Proteintech

AHR Monoclonal Antibody (2D1F9)

AHR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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AHR Antibody (67785-1-IG) in IHC (P)

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Blue (DAPI).

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Product Details

67785-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2D1F9

Immunogen

AHR Fusion Protein Ag28935

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Indole-3-propionic acid alleviates chondrocytes inflammation and osteoarthritis via the AhR/NF-κB axis.

Molecular medicine (Cambridge, Mass.)

Zhuang H,Ren X,Jiang F,Zhou P

Tue Jan 31 00:00:00 UTC 2023

Serum Response Factor-Regulated IDO1/Kyn-Ahr Pathway Promotes Tumorigenesis of Oral Squamous Cell Carcinoma.

Cancers

Xu M,Zhu F,Yin Q,Yin H,Fang S,Luo G,Huang J,Huang W,Liu F,Zhong M,Deng X

Sun Feb 19 00:00:00 UTC 2023

Type 1 diabetes and diet-induced obesity predispose C57BL/6J mice to PM_2.5-induced lung injury: a comparative study.

Particle and fibre toxicology

Chen S,Li M,Zhang R,Ye L,Jiang Y,Jiang X,Peng H,Wang Z,Guo Z,Chen L,Zhang R,Niu Y,Aschner M,Li D,Chen W

Mon Apr 17 00:00:00 UTC 2023

Crosstalk between the aryl hydrocarbon receptor (AhR) and the peroxisome proliferator-activated receptor gamma (PPARγ) as a key factor in the metabolism of silver nanoparticles in neuroblastoma (SH-SY5Y) cells in vitro.

Toxicology and applied pharmacology

Skóra B,Matuszewska P,Masicz M,Sikora K,Słomczewska M,Sołtysek P,Szychowski KA

Sun Jan 01 00:00:00 UTC 2023

Gut microbiota-derived 3-phenylpropionic acid promotes intestinal epithelial barrier function via AhR signaling.

Microbiome

Hu J,Chen J,Xu X,Hou Q,Ren J,Yan X

Mon May 08 00:00:00 UTC 2023

Glutathione peroxidase 2 knockdown suppresses gastric cancer progression and metastasis via regulation of kynurenine metabolism.

Oncogene

Xu H,Hu C,Wang Y,Shi Y,Yuan L,Xu J,Zhang Y,Chen J,Wei Q,Qin J,Xu Z,Cheng X

Thu Jun 01 00:00:00 UTC 2023

Glutathione peroxidase 2 knockdown suppresses gastric cancer progression and metastasis via regulation of kynurenine metabolism.

Oncogene

Xu H,Hu C,Wang Y,Shi Y,Yuan L,Xu J,Zhang Y,Chen J,Wei Q,Qin J,Xu Z,Cheng X

Thu Jun 01 00:00:00 UTC 2023

Knockdown of IL4I1 Improved High Glucose-evoked Insulin Resistance in HepG2 Cells by Alleviating Inflammation and Lipotoxicity Through AHR Activation.

Applied biochemistry and biotechnology

Run L,Tian Z,Xu L,Du J,Li N,Wang Q,Sun H

Wed Nov 01 00:00:00 UTC 2023

Ginsenoside Rb1 prevents osteoporosis via the AHR/PRELP/NF-κB signaling axis.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Zhang D,Du J,Yu M,Suo L

Thu Sep 01 00:00:00 UTC 2022

RAB3D, upregulated by aryl hydrocarbon receptor (AhR), promotes the progression of prostate cancer by activating the PI3K/AKT signaling pathway.

Cell biology international

Yu J,Qi H,Wang Z,Zhang Z,Song E,Song W,An R

Thu Dec 01 00:00:00 UTC 2022

MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and Fibrosis.

Journal of inflammation research