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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • AHR Antibodies

          Invitrogen

          AHR Monoclonal Antibody (FF3399), eBioscience™

          Advanced Verification
          2 Published Figures
          5 References
          View all (50) AHR antibodies

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          Cite AHR Monoclonal Antibody (FF3399), eBioscience™

          • Antibody Testing Data (5)
          • Published Figures (2)
          • Advanced Verification (4)
          AHR Antibody in Immunocytochemistry (ICC/IF)
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          AHR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          AHR Antibody (14-9854-82) in ICC/IF

          Immunofluorescence analysis of AHR was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% Paraformaldehyde for 10 Minutes, permeabilized with 0.1% Triton™ X-100 for 10 Minutes, and blocked with 2% BSA for 10 Minutes at room temperature. The cells were labeled with AHR Monoclonal Antibody (FF3399), eBioscience (Product # 14-9854-82) at 20 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Prod... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          AHR Antibody in Immunocytochemistry (ICC/IF)
          AHR Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Flow Cytometry (Flow)
          AHR Antibody
          AHR Antibody
          AHR Antibody
          AHR Antibody

          Product Details

          14-9854-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          20 µg/mL
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          20 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          20 µg/mL
          -

          Flow Cytometry (Flow)

          Assay-Dependent
          View 2 publications 2 publications

          Immunoelectrophoresis (IE)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG2b, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          FF3399

          Immunogen

          21H2-AHR (aa 449-848)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • eFluor 660
          • PE 
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2573042

          Product Specific Information

          Description: The FF3399 monoclonal antibody recognizes human aryl hydrocarbon receptor (AHR). The AHR is a ligand-activated transcription factor that mediates the toxic effects of a diverse group of environmental contaminants, most notably aryl hydrocarbons such as polychlorinated biphenyls (PCB) and tetrachlorodibenzo-p-dioxin (TCDD). The AHR has also been shown to bind to a number of naturally occurring compounds found in fruits and vegetables as well as compounds generated through normal cellular metabolism. AHR is localized in the cytoplasm in a complex that includes HSP90, p23, and XAP2/AIP/ARA9. Upon ligand-binding, AHR translocates to the nucleus and binds with aryl hydrocarbon receptor nuclear translocator (ARNT), and this complex binds to the consensus DNA sequence, GCGTG, found in the promoter/enhancer regions of many genes such as CYP1A1. The AHR is expressed in many cell types, with highest expression levels found in liver. The AHR has been shown to play a role in the regulation/differentiation of Treg and Th17 cells.

          Applications Reported: This FF3399 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry (Fluorochrome-conjugated FF3399 is recommended for use in intracellular flow cytometry).

          Applications Tested: This FF3399 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using high or low pH antigen retrieval solutions (cats. 00-4955 and 00-4956) by immunocytochemistry on fixed and permeabilized human cells, and by western blot. FF3399 can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Ah receptor; AH-receptor; aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76

          View more View less

          Gene Aliases: AHR; BHLHE76

          View more View less

          UniProt ID: (Human) P35869

          View more View less

          Entrez Gene ID: (Human) 196

          View more View less

          Function(s)
          DNA binding transcription factor activity, sequence-specific DNA binding aryl hydrocarbon receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding enhancer binding transcription regulatory region DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding E-box binding
          Process(es)
          ovarian follicle development blood vessel development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter xenobiotic metabolic process apoptotic process cell cycle response to xenobiotic stimulus response to toxic substance regulation of gene expression intracellular receptor signaling pathway regulation of B cell proliferation response to estradiol circadian regulation of gene expression negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cellular response to glucocorticoid stimulus
          It has to be done as per old AB suggested Products section.
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