Invitrogen

AHR Monoclonal Antibody (FF3399), eBioscience™

AHR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

AHR Antibody (14-9854-82) in ICC/IF

Immunofluorescence analysis of AHR was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% Paraformaldehyde for 10 Minutes, permeabilized with 0.1% Triton™ X-100 for 10 Minutes, and blocked with 2% BSA for 10 Minutes at room temperature. The cells were labeled with AHR Monoclonal Antibody (FF3399), eBioscience (Product # 14-9854-82) at 20 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Prod... View More

AHR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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AHR Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. Relative expression of AHR was observed in A-375 and U-87 MG in comparison to Jurkat and MOLT-4 using Anti- AHR Monoclonal Antibody (Product # 14-9854-82) Western Blot. {RE}

Product Details

14-9854-82

Applications

Tested Dilution

Publications

Western Blot (WB)

20 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

Immunocytochemistry (ICC/IF)

20 µg/mL

Flow Cytometry (Flow)

Assay-Dependent

View 2 publications 2 publications

Immunoelectrophoresis (IE)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

FF3399

Immunogen

21H2-AHR (aa 449-848)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2573042

Product Specific Information

Description: The FF3399 monoclonal antibody recognizes human aryl hydrocarbon receptor (AHR). The AHR is a ligand-activated transcription factor that mediates the toxic effects of a diverse group of environmental contaminants, most notably aryl hydrocarbons such as polychlorinated biphenyls (PCB) and tetrachlorodibenzo-p-dioxin (TCDD). The AHR has also been shown to bind to a number of naturally occurring compounds found in fruits and vegetables as well as compounds generated through normal cellular metabolism. AHR is localized in the cytoplasm in a complex that includes HSP90, p23, and XAP2/AIP/ARA9. Upon ligand-binding, AHR translocates to the nucleus and binds with aryl hydrocarbon receptor nuclear translocator (ARNT), and this complex binds to the consensus DNA sequence, GCGTG, found in the promoter/enhancer regions of many genes such as CYP1A1. The AHR is expressed in many cell types, with highest expression levels found in liver. The AHR has been shown to play a role in the regulation/differentiation of Treg and Th17 cells.

Applications Reported: This FF3399 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry (Fluorochrome-conjugated FF3399 is recommended for use in intracellular flow cytometry).

Applications Tested: This FF3399 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using high or low pH antigen retrieval solutions (cats. 00-4955 and 00-4956) by immunocytochemistry on fixed and permeabilized human cells, and by western blot. FF3399 can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aryl Hydrocarbon Receptor Activation Suppresses EBF1 and PAX5 and Impairs Human B Lymphopoiesis.

Journal of immunology (Baltimore, Md. : 1950)

Li J,Bhattacharya S,Zhou J,Phadnis-Moghe AS,Crawford RB,Kaminski NE

14-9854 was used in Western blot to demonstrate a role for the AHR in regulating human B cell development, and suggest that transcriptional alterations of EBF1 by the AHR are involved in the underlying mechanism.

Wed Nov 15 00:00:00 UTC 2017

The role of indoleamine 2,3-dioxygenase-aryl hydrocarbon receptor pathway in the TLR4-induced tolerogenic phenotype in human DCs.

Scientific reports

Salazar F,Awuah D,Negm OH,Shakib F,Ghaemmaghami AM

14-9854 was used in Flow cytometry/Cell sorting to show the LR4-induced tolerogenic phenotype in human dendritic cells is influenced by the tryptophan-metabolizing enzyme indoleamine 2,3-dioxygenase.

Fri Mar 03 00:00:00 UTC 2017

Role of aryl hydrocarbon receptor polymorphisms on TCDD-mediated CYP1B1 induction and IgM suppression by human B cells.

Toxicology and applied pharmacology

Kovalova N,Manzan M,Crawford R,Kaminski N

14-9854 was used in Western Blotting to investigate the functional properties of six aryl hydrocarbon receptor (AhR) variants in the human B cell line in relation to tetrachlorodibenzo-p-dioxin sensitivity.

Sat Oct 15 00:00:00 UTC 2016

Role of aryl hydrocarbon receptor polymorphisms on TCDD-mediated CYP1B1 induction and IgM suppression by human B cells.

Toxicology and applied pharmacology

Kovalova N,Manzan M,Crawford R,Kaminski N

Sat Oct 15 00:00:00 UTC 2016

SHP-1 is directly activated by the aryl hydrocarbon receptor and regulates BCL-6 in the presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

Toxicology and applied pharmacology

Phadnis-Moghe AS,Li J,Crawford RB,Kaminski NE

14-9854 was used in Electrophoretic mobility shift assay to suggest that SHP-1 is regulated by AHR and may regulate TCDD-mediated suppression of human B-cell activation.

Tue Nov 01 00:00:00 UTC 2016

Generation of IL-8 and IL-9 producing CD4⁺ T cells is affected by Th17 polarizing conditions and AHR ligands.

Mediators of inflammation

Gasch M,Goroll T,Bauer M,Hinz D,Schütze N,Polte T,Kesper D,Simon JC,Hackermüller J,Lehmann I,Herberth G

Published figure using AHR monoclonal antibody (Product # 14-9854-82) in Flow Cytometry

Mon Dec 15 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Ah receptor; AH-receptor; aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76

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Gene Aliases: AHR; BHLHE76

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UniProt ID: (Human) P35869

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Entrez Gene ID: (Human) 196

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding aryl hydrocarbon receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding enhancer binding transcription regulatory region DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding E-box binding

Process(es)

ovarian follicle development blood vessel development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter xenobiotic metabolic process apoptotic process cell cycle response to xenobiotic stimulus response to toxic substance regulation of gene expression intracellular receptor signaling pathway regulation of B cell proliferation response to estradiol circadian regulation of gene expression negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cellular response to glucocorticoid stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AHR Monoclonal Antibody (FF3399), eBioscience™

AHR Antibody (14-9854-82) in ICC/IF

Product Details

14-9854-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoelectrophoresis (IE)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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