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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • AHR Antibodies

          Invitrogen

          AHR Monoclonal Antibody (RPT1)

          Advanced Verification
          31 Published Figures
          52 References
          View all (46) AHR antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite AHR Monoclonal Antibody (RPT1)

          • Antibody Testing Data (10)
          • Published Figures (31)
          • Advanced Verification (5)
          AHR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          AHR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 46

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          AHR Antibody (MA1-514) in ICC/IF

          Immunofluorescence analysis of AHR was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with AHR Monoclonal Antibody (RPT1) (Product # MA1-514) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          AHR Antibody in Immunocytochemistry (ICC/IF)
          AHR Antibody in Immunocytochemistry (ICC/IF)
          AHR Antibody in Immunocytochemistry (ICC/IF)
          AHR Antibody in Immunocytochemistry (ICC/IF)
          AHR Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AHR Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot, Immunoprecipitation (WB, IP)
          AHR Antibody in Immunocytochemistry (ICC/IF)
          AHR Antibody in Immunocytochemistry (ICC/IF)
          AHR Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))
          AHR Antibody in Immunohistochemistry (IHC)
          AHR Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))
          AHR Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))
          AHR Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          AHR Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          AHR Antibody in Immunohistochemistry (IHC)
          AHR Antibody in Immunohistochemistry (IHC)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in Western Blot (WB)
          AHR Antibody in ChIP Assay (ChIP)
          AHR Antibody in ChIP Assay (ChIP)
          AHR Antibody
          AHR Antibody
          AHR Antibody
          AHR Antibody
          AHR Antibody

          Product Details

          MA1-514

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 35 publications 35 publications

          Immunohistochemistry (IHC)

          -
          View 5 publications 5 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:10 - 1:100
          -

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          View 3 publications 3 publications

          ELISA (ELISA)

          -
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 2 publications 2 publications

          ChIP assay (ChIP)

          -
          View 4 publications 4 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Non-human primate, Pig

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          RPT1

          Immunogen

          Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2273723

          Product Specific Information

          MA1-514 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.

          MA1-514 has been successfully used in Western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~96 kDa protein representing AhR, with some minor non-specific bands observed in certain samples.

          MA1-514 immunogen is residues 12-31 from the human AhR protein with amino acids 18-21 being omitted.

          Target Information

          AHR (Ah Receptor) belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. AHR contains an N-terminal sequence of approximately 200 amino acids termed the PAS domain. AHR, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene. The gene for AHR encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450, and its ligands included a variety of aromatic hydrocarbons. AHR is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for AHR. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form, and this transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). Diseases associated with AHR include eosinophilic fasciitis and seborrheic dermatitis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Ah receptor; AH-receptor; aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76; dioxin receptor

          View more View less

          Gene Aliases: Ah; Ahh; AHR; Ahre; BHLHE76; In

          View more View less

          UniProt ID: (Human) P35869, (Mouse) P30561

          View more View less

          Entrez Gene ID: (Human) 196, (Mouse) 11622, (Rat) 25690

          View more View less

          Function(s)
          DNA binding transcription factor activity, sequence-specific DNA binding aryl hydrocarbon receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding enhancer binding transcription regulatory region DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding E-box binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding signal transducer activity sequence-specific DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter cell morphogenesis ovarian follicle development blood vessel development patterning of blood vessels B cell homeostasis liver development B-1 B cell homeostasis blood vessel remodeling lymphocyte homeostasis immune system process negative regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis circumferential growth involved in left ventricle morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter xenobiotic metabolic process response to stress cell cycle signal transduction blood circulation regulation of blood pressure response to xenobiotic stimulus response to toxic substance regulation of gene expression response to organic cyclic compound B cell differentiation intracellular receptor signaling pathway prostate gland development regulation of B cell proliferation circadian regulation of gene expression embryonic hemopoiesis post-embryonic hemopoiesis camera-type eye development T cell homeostasis positive regulation of cell size negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly negative regulation of vasoconstriction positive regulation of growth rhythmic process blood vessel morphogenesis spleen development reproductive structure development gland development smooth muscle tissue development regulation of blood vessel size regulation of heart growth negative regulation of necrotic cell death venous blood vessel development kidney morphogenesis common bile duct development cellular response to cAMP glomerulus morphogenesis apoptotic process response to estradiol positive regulation of transcription from RNA polymerase II promoter cellular response to glucocorticoid stimulus
          It has to be done as per old AB suggested Products section.
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