Invitrogen

AID Monoclonal Antibody (mAID-2), Biotin, eBioscience™

13 Published Figures

9 References

View all (17) AID antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (13)

AID Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 14

AID Antibody (13-5959-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using low pH antigen retrieval and stained with 20 µg/mL Rat IgG2a K Isotype Control Biotin (left) or 20 µg/mL Anti-Human/Mouse Activation-Induced Cytidine Deaminase (AID) Biotin (right) followed by Avidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.

AID Antibody in Western Blot, Flow Cytometry (WB, Flow)

AID Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

13-5959-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

20 µg/mL

Flow Cytometry (Flow)

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

mAID-2

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572798

Product Specific Information

Description: The monoclonal mAID-2 recognizes human and mouse AID (gene AICDA) also known as Activation-Induced Cytidine Deaminase. AID is a 24 kDa RNA-editing enzyme that converts cytosine into uracil. It plays a critical role during B cell development, specifically in somatic hypermutation (SHM) and immunoglobulin class switch recombination (CSR). Changes in levels of AID expression typically present with disease. For example, mutations in AID are found in Hyper-IgM Syndrome (type 2), a disease form without opportunistic infections, while overexpression correlates with poor prognosis in chronic lymphocytic leukemia/small lymphocytic lymphoma.

AID is expressed mainly by activated mature B cells such as mature germinal centre (GC) B cells but also in intermediate GC cells (defined as IgD+CD38-CD23-CD71+). In addition to hematopoietic cells, AID is expressed in primary human hepatocellular carcinomas, helicobacter infected gastric epithelial cells, oocytes and embryonic stem cells although it role in these cells is not well understood. Because AID contains an NLS (nuclear localization signal), expression can be found both in the nucleus as well as in the cytoplasm.

Applications Reported: This mAID-2 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

Applications Tested: This mAID-2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal perfomance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Interplay between UNG and AID governs intratumoral heterogeneity in mature B cell lymphoma.

PLoS genetics

Delgado P,Álvarez-Prado ÁF,Marina-Zárate E,Sernandez IV,Mur SM,de la Barrera J,Sanchez-Cabo F,Cañamero M,de Molina A,Belver L,de Yébenes VG,Ramiro AR

Published figure using AID monoclonal antibody (Product # 13-5959-82) in Western Blot

Tue Dec 01 00:00:00 UTC 2020

Phosphatidylinositol 3-kinase δ blockade increases genomic instability in B cells.

Nature

Compagno M,Wang Q,Pighi C,Cheong TC,Meng FL,Poggio T,Yeap LS,Karaca E,Blasco RB,Langellotto F,Ambrogio C,Voena C,Wiestner A,Kasar SN,Brown JR,Sun J,Wu CJ,Gostissa M,Alt FW,Chiarle R

Published figure using AID monoclonal antibody (Product # 13-5959-82) in ChIP assay

Thu Feb 23 00:00:00 UTC 2017

Phosphatidylinositol 3-kinase δ blockade increases genomic instability in B cells.

Nature

Compagno M,Wang Q,Pighi C,Cheong TC,Meng FL,Poggio T,Yeap LS,Karaca E,Blasco RB,Langellotto F,Ambrogio C,Voena C,Wiestner A,Kasar SN,Brown JR,Sun J,Wu CJ,Gostissa M,Alt FW,Chiarle R

Published figure using AID monoclonal antibody (Product # 13-5959-82) in ChIP assay

Thu Feb 23 00:00:00 UTC 2017

CCCTC-Binding Factor Locks Premature IgH Germline Transcription and Restrains Class Switch Recombination.

Frontiers in immunology

Marina-Zárate E,Pérez-García A,Ramiro AR

Published figure using AID monoclonal antibody (Product # 13-5959-82) in Western Blot

Mon Jan 31 00:00:00 UTC 2022

B-cell activation with CD40L or CpG measures the function of B-cell subsets and identifies specific defects in immunodeficient patients.

European journal of immunology

Marasco E,Farroni C,Cascioli S,Marcellini V,Scarsella M,Giorda E,Piano Mortari E,Leonardi L,Scarselli A,Valentini D,Cancrini C,Duse M,Grimsholm O,Carsetti R

13-5959-82 was used in Flow Cytometry to assess the functions of the humoral immune system.

Sun Jan 01 00:00:00 UTC 2017

TGF-β suppression of HBV RNA through AID-dependent recruitment of an RNA exosome complex.

PLoS pathogens

Liang G,Liu G,Kitamura K,Wang Z,Chowdhury S,Monjurul AM,Wakae K,Koura M,Shimadu M,Kinoshita K,Muramatsu M

Published figure using AID monoclonal antibody (Product # 13-5959-82) in Immunoprecipitation

Wed Apr 01 00:00:00 UTC 2015

TGF-β suppression of HBV RNA through AID-dependent recruitment of an RNA exosome complex.

PLoS pathogens

Liang G,Liu G,Kitamura K,Wang Z,Chowdhury S,Monjurul AM,Wakae K,Koura M,Shimadu M,Kinoshita K,Muramatsu M

Published figure using AID monoclonal antibody (Product # 13-5959-82) in Immunoprecipitation

Wed Apr 01 00:00:00 UTC 2015

HSP90 inhibitors decrease AID levels and activity in mice and in human cells.

European journal of immunology

Montamat-Sicotte D,Litzler LC,Abreu C,Safavi S,Zahn A,Orthwein A,Müschen M,Oppezzo P,Muñoz DP,Di Noia JM

Published figure using AID monoclonal antibody (Product # 13-5959-82) in Western Blot

Sat Aug 01 00:00:00 UTC 2015

HSP90 inhibitors decrease AID levels and activity in mice and in human cells.

European journal of immunology

Montamat-Sicotte D,Litzler LC,Abreu C,Safavi S,Zahn A,Orthwein A,Müschen M,Oppezzo P,Muñoz DP,Di Noia JM

Published figure using AID monoclonal antibody (Product # 13-5959-82) in Western Blot

Sat Aug 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: activation induced deaminase; Activation-induced cytidine deaminase; AID; Cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos; Single-stranded DNA cytosine deaminase

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Gene Aliases: AICDA; AID; ARP2; CDA2; HEL-S-284; HIGM2

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UniProt ID: (Human) Q9GZX7, (Mouse) Q9WVE0

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Entrez Gene ID: (Human) 57379, (Mouse) 11628

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Function(s)

cytidine deaminase activity protein binding zinc ion binding ubiquitin protein ligase binding catalytic activity hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines metal ion binding

Process(es)

mRNA processing cytidine deamination somatic diversification of immunoglobulins somatic hypermutation of immunoglobulin genes B cell differentiation isotype switching cellular response to lipopolysaccharide DNA demethylation negative regulation of methylation-dependent chromatin silencing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AID Monoclonal Antibody (mAID-2), Biotin, eBioscience™

AID Antibody (13-5959-82) in IHC (P)

Product Details

13-5959-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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