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Invitrogen
AIF Monoclonal Antibody (7F7AB10)
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FIGURE: 1 / 9
AIF Antibody (45-6200) in ICC/IF
Immunofluorescence analysis of AIF was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with AIF Monoclonal Antibody (7F7AB10) (Product # 45-6200) at 5 µg in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A281... View More
Product Details
45-6200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
7F7AB10
Immunogen
Human heart mitochondria
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
IgG fraction
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533822
Product Specific Information
When performing IHC use heat mediated antigen retrieval before commencing with IHC staining protocol.
When perfoming ICC or IF recommended sample treatment are paraformaldehyde fixed at 4% for 20 minutes and Triton X-100 permeabilized at 0.1% for 15 minutes.
Target Information
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AG1; AGR1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; ER protein 18; ER protein 19; ERP18; ERP19; hAG-1; hTLP19; MGC111425; pcd8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376
Gene Aliases: AIF; AIFM1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8
UniProt ID: (Human) O95831
Entrez Gene ID: (Human) 9131
response to ischemia
DNA catabolic process
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to toxic substance
chromosome condensation
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
cell redox homeostasis
neuron apoptotic process
oxidation-reduction process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
cellular response to estradiol stimulus
cellular response to nitric oxide
cellular response to oxygen-glucose deprivation
response to L-glutamate
regulation of apoptotic DNA fragmentation
cellular response to aldosterone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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