Invitrogen

AIM2 Polyclonal Antibody, eBioscience™

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AIM2 Antibody (14-6008-93) in WB

Cell lysates prepared from Jurkat cells (lane 1) and mouse macrophages left untreated (lane 2) or treated for 12 hr with IFN gamma and LPS (lane 3) were immunoblotted with a 1:1000 dilution of the Anti-AIM2 polyclonal antibody. Bands were visualized using Anti-Rabbit IgG HRP.

AIM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AIM2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

AIM2 Antibody — Inflammasome activation in AIM2 knockdown THP-1 cells after EBV infection. The following analyses were performed at 24 hours after incubation with RPMI (mock) or AGS-EBV-GFP cell supernatant (AGSGFP). (A) AIM2 mRNA expression of siRNA-treated THP-1 cells. The asterisk (*) indicates p = 0.02 (B) Immunoblot analysis of siRNA-treated THP-1 cells. Β-actin was blotted as a loading control. (C) mRNA expression of caspase-1 and IL-1Β in siRNA-treated THP-1 cells. The double asterisk (**) indicates p<0.001 by one-way ANOVA with Tukey’s post hoc test. (D) IL-1Β concentration in siRNA-treated THP-1 cells. (A-D) The data are expressed as fold changes compared to si-control-treated THP-1 cells incubated with RPMI (mock). (A, C, D) The data represent one experiment with triplicate samples. The error bars represent S.E. {KD}

Product Details

14-6008-93

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10670279

Product Specific Information

Description: This polyclonal antibody reacts with human, mouse, and rat AIM2 (also known as Antigen isolated from Immunoselected Melanoma 2). A member of the HIN-2000 family, this 40-kDa cytoplasmic protein is expressed in the spleen, small intestine, peripheral blood lymphocytes, and testis. Moreover, AIM2 is expressed in tumors of the neuroectodermis, breast, ovary, and colon. As a component of the inflammosome, AIM2 detects double-stranded viral and bacterial DNA present in the cytosol and initiates Caspase1 activation. Expression of AIM2 can be induced by interferons.

Applications Reported: This polyclonal antibody has been reported for use in immunoblotting (WB).

Applications Tested: This polyclonal antibody has been tested by immunoblotting of Jurkat cells and mouse macrophages treated for 12 hrs with IFN gamma and LPS. This can be used at less than or equal to a 1:1000 dilution. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Methods to Study Inflammasome Activation in the Central Nervous System: Immunoblotting and Immunohistochemistry.

Methods in molecular biology (Clifton, N.J.)

Cyr B,de Rivero Vaccari JP

14-6008-93 was used in Western Blot to study inflammasome signaling in CNS tissues (brain and spinal cord).

Tue Aug 15 00:00:00 UTC 2023

Methods to Study Inflammasome Activation in the Central Nervous System: Immunoblotting and Immunohistochemistry.

Methods in molecular biology (Clifton, N.J.)

Cyr B,de Rivero Vaccari JP

14-6008-93 was used in Western Blot to study inflammasome signaling in CNS tissues (brain and spinal cord).

Tue Aug 15 00:00:00 UTC 2023

Caspase-11 interaction with NLRP3 potentiates the noncanonical activation of the NLRP3 inflammasome.

Nature immunology

Moretti J,Jia B,Hutchins Z,Roy S,Yip H,Wu J,Shan M,Jaffrey SR,Coers J,Blander JM

14-6008-93 was used in Western Blot to find that bacterial mRNA, which signals bacterial viability, was required alongside LPS for noncanonical activation of the NLRP3 inflammasome in macrophages.

Sun May 01 00:00:00 UTC 2022

Caspase-11 interaction with NLRP3 potentiates the noncanonical activation of the NLRP3 inflammasome.

Nature immunology

Moretti J,Jia B,Hutchins Z,Roy S,Yip H,Wu J,Shan M,Jaffrey SR,Coers J,Blander JM

14-6008-93 was used in Western Blot to find that bacterial mRNA, which signals bacterial viability, was required alongside LPS for noncanonical activation of the NLRP3 inflammasome in macrophages.

Sun May 01 00:00:00 UTC 2022

BECN2 (beclin 2) Negatively Regulates Inflammasome Sensors Through ATG9A-Dependent but ATG16L1- and LC3-Independent Non-Canonical Autophagy.

Autophagy

Deng G,Li C,Chen L,Xing C,Fu C,Qian C,Liu X,Wang HY,Zhu M,Wang RF

14-6008-93 was used in Immunoprecipitation, Western Blot assay to study the role of beclin 2 on regulation of inflammasome sensors.

Tue Feb 01 00:00:00 UTC 2022

BECN2 (beclin 2) Negatively Regulates Inflammasome Sensors Through ATG9A-Dependent but ATG16L1- and LC3-Independent Non-Canonical Autophagy.

Autophagy

Deng G,Li C,Chen L,Xing C,Fu C,Qian C,Liu X,Wang HY,Zhu M,Wang RF

14-6008-93 was used in Immunoprecipitation, Western Blot assay to study the role of beclin 2 on regulation of inflammasome sensors.

Tue Feb 01 00:00:00 UTC 2022

Inhibition of AIM2 inflammasome activation alleviates GSDMD-induced pyroptosis in early brain injury after subarachnoid haemorrhage.

Cell death & disease

Yuan B,Zhou XM,You ZQ,Xu WD,Fan JM,Chen SJ,Han YL,Wu Q,Zhang X

14-6008 was used in Western Blotting to show that the inhibition of AIM2 inflammasome activation caused by knocking down AIM2 and caspase-1 alleviates GSDMD-induced pyroptosis in EBI after SAH.

Thu Jan 30 00:00:00 UTC 2020

Inhibition of AIM2 inflammasome activation alleviates GSDMD-induced pyroptosis in early brain injury after subarachnoid haemorrhage.

Cell death & disease

Yuan B,Zhou XM,You ZQ,Xu WD,Fan JM,Chen SJ,Han YL,Wu Q,Zhang X

14-6008 was used in Western Blotting to show that the inhibition of AIM2 inflammasome activation caused by knocking down AIM2 and caspase-1 alleviates GSDMD-induced pyroptosis in EBI after SAH.

Thu Jan 30 00:00:00 UTC 2020

MARK4 (Microtubule Affinity-Regulating Kinase 4)-Dependent Inflammasome Activation Promotes Atherosclerosis-Brief Report.

Arteriosclerosis, thrombosis, and vascular biology

Clement M,Chen X,Chenoweth HL,Teng Z,Thome S,Newland SA,Harrison J,Yu X,Finigan AJ,Mallat Z,Li X

14-6008-93 was used in Immunohistochemistry to study the role of MARK4 in hematopoietic cells during atherosclerosis.

Thu Aug 01 00:00:00 UTC 2019

The PYHIN Protein p205 Regulates the Inflammasome by Controlling Asc Expression.

Journal of immunology (Baltimore, Md. : 1950)

Ghosh S,Wallerath C,Covarrubias S,Hornung V,Carpenter S,Fitzgerald KA

14-6008 was used in Western Blotting to identify an immune-regulatory function for a member of the murine PYHIN protein family, p205.

Wed Nov 01 00:00:00 UTC 2017

Epstein-Barr virus infection-induced inflammasome activation in human monocytes.

PloS one

Torii Y,Kawada JI,Murata T,Yoshiyama H,Kimura H,Ito Y

14600893 was used in western blot to study inflammasome activation during primary Epstein-Barr virus infection

Tue Sep 12 00:00:00 UTC 2017

Epstein-Barr virus infection-induced inflammasome activation in human monocytes.

PloS one

Torii Y,Kawada JI,Murata T,Yoshiyama H,Kimura H,Ito Y

14600893 was used in western blot to study inflammasome activation during primary Epstein-Barr virus infection

Tue Sep 12 00:00:00 UTC 2017

Epstein-Barr virus infection-induced inflammasome activation in human monocytes.

PloS one

Torii Y,Kawada JI,Murata T,Yoshiyama H,Kimura H,Ito Y

14600893 was used in western blot to study inflammasome activation during primary Epstein-Barr virus infection

Tue Sep 12 00:00:00 UTC 2017

Acidification changes affect the inflammasome in human nucleus pulposus cells.

Journal of inflammation (London, England)

Brand FJ,Forouzandeh M,Kaur H,Travascio F,de Rivero Vaccari JP

14-6008 was used in Western Blotting to suggest the inflammatory response through IL-1β in the human disc is not initiated in nucleus pulposus cells when stimulated with acidification.

Fri Aug 26 00:00:00 UTC 2016

Expression analysis of inflammasomes in experimental models of inflammatory and fibrotic liver disease.

Journal of inflammation (London, England)

Boaru SG,Borkham-Kamphorst E,Tihaa L,Haas U,Weiskirchen R

14-6008 was used in Immunohistochemistry on paraffin embedded tissues to investigate the expression of the different inflammasomes in various primary hepatic cell subpopulations.

Wed Nov 28 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Absent in melanoma 2; Ifi-210; Interferon-inducible protein 210; Interferon-inducible protein AIM2; Interferon-inducible protein p210; RP11-520H16.3

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Gene Aliases: AIM2; Gm1313; Ifi210; PYHIN4

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UniProt ID: (Human) O14862, (Mouse) Q91VJ1

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Entrez Gene ID: (Human) 9447, (Rat) 304987, (Mouse) 383619

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Function(s)

double-stranded DNA binding protein binding identical protein binding molecular_function DNA binding

Process(es)

activation of innate immune response positive regulation of defense response to virus by host biological_process negative regulation of NF-kappaB transcription factor activity positive regulation of protein oligomerization positive regulation of interleukin-1 beta production tumor necrosis factor-mediated signaling pathway cellular response to interferon-beta cellular response to drug interleukin-1 beta secretion positive regulation of interleukin-1 beta secretion positive regulation of NF-kappaB transcription factor activity pyroptosis positive regulation of cysteine-type endopeptidase activity immune system process apoptotic process inflammatory response innate immune response immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AIM2 Polyclonal Antibody, eBioscience™

AIM2 Antibody (14-6008-93) in WB

Product Details

14-6008-93

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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