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Rockland
AJUBA Polyclonal Antibody
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FIGURE: 1 / 2
AJUBA Antibody (600-401-854) in WB
Western blot using Rocklands Affinity Purified anti-Ajuba antibody shows detection of a 57-kDa band consistent with the expected MW for Ajuba (arrowhead). Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A431, 4) Jurkat and 5) 293 whole cell lysates. Immunoprecipitation of Ajuba followed by western blotting may result in cleaner background staining. Approximately 5 µg of each preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Ajuba antibody. Detection occurre... View More
Product Details
600-401-854
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 224-239 of Human Ajuba.
Conjugate
Form
Liquid
Concentration
1.67 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human Ajuba. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, mouse and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with Ajuba protein homologues from other sources has not been determined.
Target Information
AJUBA (Ajuba LIM Protein) is a Protein Coding gene. Diseases associated with AJUBA include Wilson-Turner X-Linked Mental Retardation Syndrome and Syndromic X-Linked Intellectual Disability. Among its related pathways are Hippo signaling pathway and Adhesion. Gene Ontology (GO) annotations related to this gene include chromatin binding and actin filament binding. An important paralog of this gene is LIMD1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: jub, ajuba homolog; LIM domain-containing protein ajuba
Gene Aliases: AJUBA; JUB
UniProt ID: (Human) Q96IF1
Entrez Gene ID: (Human) 84962
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
response to hypoxia
transcription, DNA-templated
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
lamellipodium assembly
regulation of cell migration
positive regulation of cellular biosynthetic process
positive regulation of protein complex assembly
negative regulation of kinase activity
cellular protein localization
gene silencing by miRNA
wound healing, spreading of epidermal cells
negative regulation of hippo signaling
regulation of GTPase activity
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAP kinase activity
glycerophospholipid biosynthetic process
focal adhesion assembly
regulation of cellular response to hypoxia
positive regulation of gene silencing by miRNA
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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